ArticleThe Journal of general physiology2004
Gating transitions in bacterial ion channels measured at 3 microns resolution.
Article in The Journal of general physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Asymmetric Nature of MscL Opening Revealed by Molecular Dynamics Simulations.Journal of chemical information and modeling · 2025Article
- Mechanosensitive channel MscL gating transitions coupling with constriction point shift.Protein science : a publication of the Protein Society · 2024Article
- MscS inactivation and recovery are slow voltage-dependent processes sensitive to interactions with lipids.Biophysical journal · 2024Article
- Tight hydrophobic core and flexible helices yield MscL with a high tension gating threshold and a membrane area mechanical strain buffer.Frontiers in chemistry · 2023Article
- On Bottlenecks, Helmholtz and Hearing.Function (Oxford, England) · 2022Article
- Article
- Mechanical Activation of MscL Revealed by a Locally Distributed Tension Molecular Dynamics Approach.Biophysical journal · 2021Article
- The OpenPicoAmp-100k: an open-source high-performance amplifier for single channel recording in planar lipid bilayers.Pflugers Archiv : European journal of physiology · 2019Article
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- Single-channel recordings of RyR1 at microsecond resolution in CMOS-suspended membranes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Organelle membrane derived patches: reshaping classical methods for new targets.Scientific reports · 2017Article
- Channel disassembled: Pick, tweak, and soak parts to soften.Channels (Austin, Tex.) · 2017Article
- Measurement of DNA Translocation Dynamics in a Solid-State Nanopore at 100 ns Temporal Resolution.Nano letters · 2016Article
- Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore.PLoS biology · 2016Article
- Computational modeling of neurons: intensity-duration relationship of extracellular electrical stimulation for changes in intracellular calcium.Journal of neurophysiology · 2016Article
- The role of lipids in mechanosensation.Nature structural & molecular biology · 2015Article
- Hidden Markov analysis of improved bandwidth mechanosensitive ion channel data.European biophysics journal : EBJ · 2015Article
- Molecular dynamics study on protein-water interplay in the mechanogating of the bacterial mechanosensitive channel MscL.European biophysics journal : EBJ · 2015Article
- Feeling the hidden mechanical forces in lipid bilayer is an original sense.Proceedings of the National Academy of Sciences of the United States of America · 2014Review
- Bacterial mechanosensitive channels: progress towards an understanding of their roles in cell physiology.Current opinion in microbiology · 2014Review
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2 authors.
Funding
Abstract
Ion channels of high conductance (>200 pS) are widespread among prokaryotes and eukaryotes. Two examples, the Escherichia coli mechanosensitive ion channels Ec-MscS and Ec-MscL, pass currents of 125-300 pA. To resolve temporal details of conductance transitions, a patch-clamp setup was optimized for low-noise recordings at a time resolution of 3 microns (10-20 times faster than usual). Analyses of the high-resolution recordings confirm that Ec-MscL visits many subconductance states and show that most of the intersubstate transitions occur more slowly than the effective resolution of 3 micros. There is a clear trend toward longer transition times for the larger transitions. In Ec-MscS recordings, the majority of the observed full conductance transitions are also composite. We detected a short-lived (approximately 20 microns) Ec-MscS substate at 2/3 of full conductance; transitions between 2/3 and full conductance did not show fine structure and had a time course limited by the achieved resolution. Opening and closing transitions in MscS are symmetrical and are not preceded or followed by smaller, rapid currents ("anticipations" or "regrets"). Compared with other, lower-conductance channels, these measurements may detect unusually early states in the transitions from fully closed to fully open. Increased temporal resolution at the single-molecule level reveals that some elementary steps of structural transitions are composite and follow several alternative pathways, while others still escape resolution. High-bandwidth, low-noise single-channel measurements may provide details about state transitions in other high-conductance channels; and similar procedures may also be applied to channel- and nanopore-based single-molecule DNA measurements.
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