ArticleInvestigative ophthalmology & visual science2004
Temporal changes in gene expression after injury in the rat retina.
Article in Investigative ophthalmology & visual science, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 1 of them a synthesis that pooled it.
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Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 113 citations in OpenAlex.
- Association of high myopia with crystallin beta A4 (CRYBA4) gene polymorphisms in the linkage-identified MYP6 locus.PloS one · 2012Pooled it
- Multi-genomics data mining identified critical transcription factors and immune response pathways in traumatic optic nerve injury and retinal injury.Frontiers in ophthalmology · 2026Article
- Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes.Scientific reports · 2025Article
- HSPB4/CRYAA Protect Photoreceptors during Retinal Detachment in Part through FAIM2 Regulation.Neurology international · 2024Article
- Proteomic analysis of diabetic retinas.Frontiers in endocrinology · 2023Article
- Release of VAMP5-positive extracellular vesicles by retinal Müller glia in vivo.Journal of extracellular vesicles · 2022Article
- Crystallins Play a Crucial Role in Glaucoma and Promote Neuronal Cell Survival in an In Vitro Model Through Modulating Müller Cell Secretion.Investigative ophthalmology & visual science · 2022Article
- αA-Crystallin Mediated Neuroprotection in the Retinal Neurons Is Independent of Protein Kinase B.Frontiers in neuroscience · 2022Article
- Therapeutic Potential of α-Crystallins in Retinal Neurodegenerative Diseases.Antioxidants (Basel, Switzerland) · 2021Review
- Commonalities of optic nerve injury and glaucoma-induced neurodegeneration: Insights from transcriptome-wide studies.Experimental eye research · 2021Review
- Article
- Proteomic profile of vitreous in patients with tubercular uveitis.Tuberculosis (Edinburgh, Scotland) · 2021Article
- Cd9 Protects Photoreceptors from Injury and Potentiates Edn2 Expression.Investigative ophthalmology & visual science · 2020Article
- Significant upregulation of small heat shock protein αA-crystallin in retinal detachment.Experimental eye research · 2019Article
- Localization of αA-Crystallin in Rat Retinal Müller Glial Cells and Photoreceptors.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2018Article
- Role of Inflammation in Diabetic Retinopathy.International journal of molecular sciences · 2018Review
- Recombination and identification of human alpha B-crystallin.International journal of ophthalmology · 2018Article
- Retinal genes are differentially expressed in areas of primary versus secondary degeneration following partial optic nerve injury.PloS one · 2018Article
- The proteomic profile of a mouse model of proliferative vitreoretinopathy.FEBS open bio · 2017Article
- Enhancing the efficacy of AREDS antioxidants in light-induced retinal degeneration.Molecular vision · 2017Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe goal of this study was to define the temporal changes in gene expression after retinal injury and to relate these changes to the inflammatory and reactive response. A specific emphasis was placed on the tetraspanin family of proteins and their relationship with markers of reactive gliosis.
methodsRetinal tears were induced in adult rats by scraping the retina with a needle. After different survival times (4 hours, and 1, 3, 7, and 30 days), the retinas were removed, and mRNA was isolated, prepared, and hybridized to the Affymatrix RG-U34A microarray (Santa Clara, CA). Microarray results were confirmed by using RT-PCR and correlation to protein levels was determined.
resultsOf the 8750 genes analyzed, approximately 393 (4.5%) were differentially expressed. Clustering analysis revealed three major profiles: (1) The early response was characterized by the upregulation of transcription factors; (2) the delayed response included a high percentage of genes related to cell cycle and cell death; and (3) the late, sustained profile clustered a significant number of genes involved in retinal gliosis. The late, sustained cluster also contained the upregulated crystallin genes. The tetraspanins Cd9, Cd81, and Cd82 were also associated with the late, sustained response.
conclusionsThe use of microarray technology enables definition of complex genetic changes underlying distinct phases of the cellular response to retinal injury. The early response clusters genes associate with the transcriptional regulation of the wound-healing process and cell death. Most of the genes in the late, sustained response appear to be associated with reactive gliosis.
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