ArticleRNA (New York, N.Y.)2004
Functional analysis of mRNA scavenger decapping enzymes.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 83 citations in OpenAlex.
- Biochemical and functional characterization of orf virus decapping protein OV71.Virology journal · 2025Article
- Analysis of ligand binding mechanism by dimeric receptors using stopped-flow fluorimetry-application to the human decapping scavenger enzyme.European biophysics journal : EBJ · 2025Article
- Trinucleotide cap analogs with triphosphate chain modifications: synthesis, properties, and evaluation as mRNA capping reagents.Nucleic acids research · 2024Article
- The eukaryotic translation initiation factor eIF4E elevates steady-state mProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Molecular basis of the selective processing of short mRNA substrates by the DcpS mRNA decapping enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Effect of the His-Tag Location on Decapping Scavenger Enzymes and Their Hydrolytic Activity toward Cap Analogs.ACS omega · 2020Article
- Synthesis of Trifluoromethylated Purine Ribonucleotides and Their Evaluation asThe Journal of organic chemistry · 2020Article
- A Benzophenone-Based Photocaging Strategy for the N7 Position of Guanosine.Angewandte Chemie (International ed. in English) · 2020Article
- Decapping Scavenger Enzyme Activity toward N2-Substituted 5' End mRNA Cap Analogues.ACS omega · 2019Article
- The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping.Scientific reports · 2019Article
- Crystal Structure of Histidine Triad Nucleotide-Binding Protein from the Pathogenic FungusMolecules and cells · 2019Article
- mRNAs biotinylated within the 5' cap and protected against decapping: new tools to capture RNA-protein complexes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Hydrolytic activity of human Nudt16 enzyme on dinucleotide cap analogs and short capped oligonucleotides.RNA (New York, N.Y.) · 2018Article
- mRNA cap analogues substituted in the tetraphosphate chain with CX2: identification of O-to-CCl2 as the first bridging modification that confers resistance to decapping without impairing translation.Nucleic acids research · 2017Article
- Applications of Phosphate Modification and Labeling to Study (m)RNA Caps.Topics in current chemistry (Cham) · 2017Review
- Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex.RNA (New York, N.Y.) · 2016Article
- The human decapping scavenger enzyme DcpS modulates microRNA turnover.Scientific reports · 2015Article
- An excess of catalytically required motions inhibits the scavenger decapping enzyme.Nature chemical biology · 2015Article
- Proteins involved in the degradation of cytoplasmic mRNA in the major eukaryotic model systems.RNA biology · 2014Review
- Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Eukaryotic cells primarily utilize exoribonucleases and decapping enzymes to degrade their mRNA. Two major decapping enzymes have been identified. The hDcp2 protein catalyzes hydrolysis of the 5' cap linked to an RNA moiety, whereas the scavenger decapping enzyme, DcpS, functions on a cap structure lacking the RNA moiety. DcpS is a member of the histidine triad (HIT) family of hydrolases and catalyzes the cleavage of m7GpppN. HIT proteins are homodimeric and contain two conserved 100-amino-acid HIT fold domains with independent active sites that are each sufficient to bind and hydrolyze cognate substrates. We carried out a functional characterization of the DcpS enzyme and demonstrate that unlike previously described HIT proteins, DcpS is a modular protein that requires both the core HIT fold at the carboxyl-terminus and sequences at the amino-terminus of the protein for cap binding and hydrolysis. Interestingly, DcpS can efficiently compete for and hydrolyze the cap structure even in the presence of excess eIF4E, implying that DcpS could function to alleviate the accumulation of complexes between eIF4E and cap structure that would otherwise accumulate following mRNA decay. Using immunofluorescence microscopy, we demonstrate that DcpS is predominantly a nuclear protein, with low levels of detected protein in the cytoplasm. Furthermore, analysis of the endogenous hDcp2 protein reveals that in addition to the cytoplasmic foci, it is also present in the nucleus. These data reveal that both decapping enzymes are contained in the nuclear compartment, indicating that they may fulfill a greater function in the nucleus than previously appreciated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.