ArticleAntimicrobial agents and chemotherapy2004
Nitric oxide generated from isoniazid activation by KatG: source of nitric oxide and activity against Mycobacterium tuberculosis.
Article in Antimicrobial agents and chemotherapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 86 citations in OpenAlex.
- Article
- Genetic Analysis of Molecular Mechanisms of Drug Resistance inInfection and drug resistance · 2025Review
- Spermine enhances the activity of anti-tuberculosis drugs.Microbiology spectrum · 2024Article
- Current Market Potential and Prospects of Copper-based Pyridine Derivatives: A Review.Current molecular medicine · 2024Review
- Isoniazid-historical development, metabolism associated toxicity and a perspective on its pharmacological improvement.Frontiers in pharmacology · 2024Review
- In vitro Antibacterial Effect Study of Plasma-Activated Saline onInfection and drug resistance · 2024Article
- Mutations and insights into the molecular mechanisms of resistance of Mycobacterium tuberculosis to first-line.Genetics and molecular biology · 2023Article
- Proteome Profiling ofACS omega · 2022Article
- Drug Discovery forInternational journal of molecular sciences · 2021Review
- Nitric Oxide-Dependent Electron Transport Chain Inhibition by the CytochromeAntimicrobial agents and chemotherapy · 2021Article
- From infection niche to therapeutic target: the intracellular lifestyle ofMicrobiology (Reading, England) · 2021Review
- Isoniazid Conjugated Magnetic Nanoparticles Loaded with Amphotericin B as a Potent Antiamoebic Agent againstAntibiotics (Basel, Switzerland) · 2020Article
- Isoniazid causes heart looping disorder in zebrafish embryos by the induction of oxidative stress.BMC pharmacology & toxicology · 2020Article
- Isoniazid and host immune system interactions: A proposal for a novel comprehensive mode of action.British journal of pharmacology · 2019Review
- Article
- Does Concurrent Use of Some Botanicals Interfere with Treatment of Tuberculosis?Neuromolecular medicine · 2016Review
- Novel 1,4-substituted-1,2,3-triazoles as antitubercular agents.ChemMedChem · 2015Article
- The future for early-stage tuberculosis drug discovery.Future microbiology · 2015Review
- Proteome turnover in bacteria: current status for Corynebacterium glutamicum and related bacteria.Microbial biotechnology · 2013Review
- Isoniazid inhibits the heme-based reactivity of Mycobacterium tuberculosis truncated hemoglobin N.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Isonicotinic acid hydrazide (INH) is a frontline antituberculosis agent. Once taken up by Mycobacterium tuberculosis, INH requires activation by the catalase-peroxidase KatG, converting INH from its prodrug form into a range of bactericidal reactive species. Here we used 15N-labeled INH together with electron paramagnetic resonance spin trapping techniques to demonstrate that nitric oxide (NO*) is generated from oxidation at the hydrazide nitrogens during the activation of INH by M. tuberculosis KatG. We also observed that a specific scavenger of NO* provided protection against the antimycobacterial activity of INH in bacterial culture. No significant increases in mycobacterial protein nitration were detected, suggesting that NOdot; and not peroxynitrite, a nitrating metabolite of NO*, is involved in antimycobacterial action. In conclusion, INH-derived NO* has biological activity, which directly contributes to the antimycobacterial action of INH.
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