ArticleBiological procedures online
Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers.
Article in Biological procedures online. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens.PloS one · 2014Article
- Myo/Nog cell regulation of bone morphogenetic protein signaling in the blastocyst is essential for normal morphogenesis and striated muscle lineage specification.Developmental biology · 2011Article
- Unique precursors for the mesenchymal cells involved in injury response and fibrosis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Noggin producing, MyoD-positive cells are crucial for eye development.Developmental biology · 2009Article
- Cells that express MyoD mRNA in the epiblast are stably committed to the skeletal muscle lineage.The Journal of cell biology · 2007Article
- MyoD-positive epiblast cells regulate skeletal muscle differentiation in the embryo.The Journal of cell biology · 2006Article
- Near-infrared laser illumination transforms the fluorescence absorbing X-Gal reaction product BCI into a transparent, yet brightly fluorescent substance.Brain research bulletin · 2006Article
- Smaller inner ear sensory epithelia in Neurog 1 null mice are related to earlier hair cell cycle exit.Developmental dynamics : an official publication of the American Association of Anatomists · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
In situ hybridization with 3DNA trade mark dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription factor MyoD in the early chick embryo. A small population of MyoD mRNA positive cells was found in the epiblast prior to the initiation of gastrulation, two days earlier than previously detected using enzymatic or radiolabeled probes for mRNA. When isolated from the epiblast and placed in culture, the MyoD mRNA positive cells were able to differentiate into skeletal muscle cells. These results demonstrate that DNA dendrimers are sensitive and precise tools for identifying low levels of mRNA in single cells and tissues.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.