ArticleThe Journal of physiology2004
Role of adenosine transport in gestational diabetes-induced L-arginine transport and nitric oxide synthesis in human umbilical vein endothelium.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 95 citations in OpenAlex.
- Potential role of A2B adenosine receptors on proliferation/migration of fetal endothelium derived from preeclamptic pregnancies.BioMed research international · 2014Trial
- A conceptual review of intracellular pH as a regulator of the adenosine-L-arginine-nitric oxide (ALANO) axis in human fetoplacental endothelial dysfunction in gestational diabetes mellitus.Pflugers Archiv : European journal of physiology · 2026Review
- Adenosine signaling: a potential therapeutic target for psychogenic erectile dysfunction.American journal of translational research · 2024Review
- TGFJournal of diabetes research · 2024Article
- Gestational Diabetes-Placental Expression of Human Equilibrative Nucleoside Transporter 1 (hENT1): Is Delayed Villous Maturation an Adaptive Pattern?Diagnostics (Basel, Switzerland) · 2023Article
- Predictive Gestational Diabetes Biomarkers With Sustained Alterations Throughout Pregnancy.Journal of the Endocrine Society · 2022Review
- Insulin regulates arginine-stimulated insulin secretion in humans.Metabolism: clinical and experimental · 2022Article
- Metabolomics signatures associated with an oral glucose challenge in pregnant women.Diabetes & metabolism · 2019Article
- Identification of a Genomic Region betweenClinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- Insulin Receptor Isoforms in Physiology and Disease: An Updated View.Endocrine reviews · 2017Review
- Insulin requires A1 adenosine receptors expression to reverse gestational diabetes-increased L-arginine transport in human umbilical vein endothelium.Purinergic signalling · 2016Article
- Cardiovascular Action of Insulin in Health and Disease: Endothelial L-Arginine Transport and Cardiac Voltage-Dependent Potassium Channels.Frontiers in physiology · 2016Review
- Adenosine signalling in diabetes mellitus--pathophysiology and therapeutic considerations.Nature reviews. Endocrinology · 2015Review
- Is a low level of free thyroxine in the maternal circulation associated with altered endothelial function in gestational diabetes?Frontiers in pharmacology · 2014Review
- Roles of nitric oxide and asymmetric dimethylarginine in pregnancy and fetal programming.International journal of molecular sciences · 2012Review
- The placenta and gestational diabetes mellitus.Current diabetes reports · 2012Review
- Insulin-increased L-arginine transport requires A(2A) adenosine receptors activation in human umbilical vein endothelium.PloS one · 2012Article
- Article
- Fetoplacental vascular endothelial dysfunction as an early phenomenon in the programming of human adult diseases in subjects born from gestational diabetes mellitus or obesity in pregnancy.Experimental diabetes research · 2011Review
- Chronic hyperglicemia and nitric oxide bioavailability play a pivotal role in pro-atherogenic vascular modifications.Genes & nutrition · 2007Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gestational diabetes is associated with increased L-arginine transport and nitric oxide (NO) synthesis, and reduced adenosine transport in human umbilical vein endothelial cells (HUVEC). Adenosine increases endothelial L-arginine/NO pathway via A(2) purinoceptors in HUVEC from normal pregnancies. It is unknown whether the effect of gestational diabetes is associated with activation of these purinoceptors or altered expression of human cationic amino acid transporter 1 (hCAT-1) or human equilibrative nucleoside transporter 1 (hENT1), or endothelial NO synthase (eNOS) in HUVEC. Cells were isolated from normal or gestational diabetic pregnancies and cultured up to passage 2. Gestational diabetes increased hCAT-1 mRNA expression (2.4-fold) and activity, eNOS mRNA (2.3-fold), protein level (2.1-fold), and phosphorylation (3.8-fold), but reduced hENT1 mRNA expression (32%) and activity. Gestational diabetes increased extracellular adenosine (2.7 microM), and intracellular L-arginine (1.9 mM) and L-citrulline (0.7 mM) levels compared with normal cells (0.05 microM, 0.89 mM, 0.35 mM, respectively). Incubation of HUVEC from normal pregnancies with 1 microM nitrobenzylthioinosine (NBMPR) mimicked the effect of gestational diabetes, but NBMPR was ineffective in diabetic cells. Gestational diabetes and NBMPR effects involved eNOS, PKC and p42/44(mapk) activation, and were blocked by the A(2a) purinoceptor antagonist ZM-241385. Thus, gestational diabetes increases the L-arginine/NO pathway involving activation of mitogen-activated protein (MAP) kinases, protein kinase C (PKC) and NO cell signalling cascades following activation of A(2a) purinoceptors by extracellular adenosine. A functional relationship is proposed between adenosine transport and modulation of L-arginine transport and NO synthesis in HUVEC, which could be determinant in regulating vascular reactivity in diabetes mellitus.
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