ArticleInfection and immunity2004
Disruption of the Aspergillus fumigatus gene encoding nucleolar protein CgrA impairs thermotolerant growth and reduces virulence.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
71 citing papers in PubMed, 135 citations in OpenAlex.
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- Retracing the evolution ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Accelerating the understanding ofCurrent research in microbial sciences · 2024Review
- The Unknown within the Known: Nucleolus, Understudied Compartment in the Filamentous Fungi.Mycobiology · 2024Review
- Pathogenicity and virulence ofVirulence · 2023Review
- Mutational Analysis ofJournal of fungi (Basel, Switzerland) · 2023Article
- Assessing thermal adaptation of a global sample ofFrontiers in public health · 2023Article
- Aspergillus Utilizes Extracellular Heme as an Iron Source During Invasive Pneumonia, Driving Infection Severity.The Journal of infectious diseases · 2022Article
- Salt stress improves thermotolerance and high-temperature bioethanol production of multi-stress-tolerant Pichia kudriavzevii by stimulating intracellular metabolism and inhibiting oxidative damage.Biotechnology for biofuels · 2021Article
- Pleiotropy and epistasis within and between signaling pathways defines the genetic architecture of fungal virulence.PLoS genetics · 2021Article
- Transcriptome profiling reveals insertional mutagenesis suppressed the expression of candidate pathogenicity genes in honeybee fungal pathogen, Ascosphaera apis.Scientific reports · 2020Article
- Molecular Mechanisms of Conidial Germination inMicrobiology and molecular biology reviews : MMBR · 2020Review
- Differential requirements of protein geranylgeranylation for the virulence of human pathogenic fungi.Virulence · 2019Article
- Investigation ofCurrent medical mycology · 2019Article
- Article
- Regulation of the heat shock transcription factor Hsf1 in fungi: implications for temperature-dependent virulence traits.FEMS yeast research · 2018Review
- Animal Models of Aspergillosis.Comparative medicine · 2018Review
- Molecular Insights Into Development and Virulence Determinants ofFrontiers in cellular and infection microbiology · 2018Review
11 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Aspergillus fumigatus CgrA is the ortholog of a yeast nucleolar protein that functions in ribosome synthesis. To determine how CgrA contributes to the virulence of A. fumigatus, a Delta cgrA mutant was constructed by targeted gene disruption, and the mutant was reconstituted to wild type by homologous introduction of a functional cgrA gene. The Delta cgrA mutant had the same growth rate as the wild type at room temperature. However, when the cultures were incubated at 37 degrees C, a condition that increased the growth rate of the wild-type and reconstituted strains approximately threefold, the Delta cgrA mutant was unable to increase its growth rate. The absence of cgrA function caused a delay in both the onset and rate of germination at 37 degrees C but had little effect on germination at room temperature. The Delta cgrA mutant was significantly less virulent than the wild-type or reconstituted strain in immunosuppressed mice and was associated with smaller fungal colonies in lung tissue. However, this difference was less pronounced in a Drosophila infection model at 25 degrees C, which correlated with the comparable growth rates of the two strains at this temperature. To determine the intracellular localization of CgrA, the protein was tagged at the C terminus with green fluorescent protein, and costaining with propidium iodide revealed a predominantly nucleolar localization of the fusion protein in living hyphae. Together, these findings establish the intracellular localization of CgrA in A. fumigatus and demonstrate that cgrA is required for thermotolerant growth and wild-type virulence of the organism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.