ArticleInfection and immunity2004
Pneumocystis carinii cell wall biosynthesis kinase gene CBK1 is an environmentally responsive gene that complements cell wall defects of cbk-deficient yeast.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Guidelines for the naming of genes, gene products, and mutants in the opportunistic protists.The Journal of eukaryotic microbiologyGuideline
- Characterization of N-Acetylglucosamine Biosynthesis in Pneumocystis species. A New Potential Target for Therapy.American journal of respiratory cell and molecular biology · 2017Article
- Regulation of Neurospora crassa cell wall remodeling via the cot-1 pathway is mediated by gul-1.Current genetics · 2017Article
- Evidence for a Pneumocystis carinii Flo8-like transcription factor: insights into organism adhesion.Medical microbiology and immunology · 2016Article
- Evidence for Proinflammatory β-1,6 Glucans in the Pneumocystis carinii Cell Wall.Infection and immunity · 2015Article
- Functional characterization of the Pneumocystis jirovecii potential drug targets dhfs and abz2 involved in folate biosynthesis.Antimicrobial agents and chemotherapy · 2015Article
- The Pneumocystis Ace2 transcription factor regulates cell wall-remodeling genes and organism virulence.The Journal of biological chemistry · 2013Article
- Chitinases in Pneumocystis carinii pneumonia.Medical microbiology and immunology · 2012Article
- The RAM network in pathogenic fungi.Eukaryotic cell · 2012Review
- Pneumocystis carinii interactions with lung epithelial cells and matrix proteins induce expression and activity of the PcSte20 kinase with subsequent phosphorylation of the downstream cell wall biosynthesis kinase PcCbk1.Infection and immunity · 2011Article
- Substrate analysis of the Pneumocystis carinii protein kinases PcCbk1 and PcSte20 using yeast proteome microarrays provides a novel method for Pneumocystis signalling biology.Yeast (Chichester, England) · 2011Article
- Pneumocystis carinii expresses an active Rtt109 histone acetyltransferase.American journal of respiratory cell and molecular biology · 2011Article
- Characterization of PCEng2, a {beta}-1,3-endoglucanase homolog in Pneumocystis carinii with activity in cell wall regulation.American journal of respiratory cell and molecular biology · 2010Article
- Characterization of the PcCdc42 small G protein from Pneumocystis carinii, which interacts with the PcSte20 life cycle regulatory kinase.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- The Pneumocystis meiotic PCRan1p kinase exhibits unique temperature-regulated activity.American journal of respiratory cell and molecular biology · 2009Article
- C1q tumor necrosis factor alpha-related protein isoform 5 is increased in mitochondrial DNA-depleted myocytes and activates AMP-activated protein kinase.The Journal of biological chemistry · 2009Article
- Characterization of a novel ADAM protease expressed by Pneumocystis carinii.Infection and immunity · 2009Article
- Pneumocystis carinii exhibits a conserved meiotic control pathway.Infection and immunity · 2008Article
- Conserved elements of the RAM signaling pathway establish cell polarity in the basidiomycete Cryptococcus neoformans in a divergent fashion from other fungi.Molecular biology of the cell · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Pneumocystis species remain an important cause of life-threatening pneumonia in immunocompromised hosts, including those with AIDS. Responses of the organism to environmental cues both within the lung and elsewhere have been poorly defined. Herein, we report the identification of a cell wall biosynthesis kinase gene (CBK1) homologue in Pneumocystis carinii, isolated by differential display PCR, that is expressed optimally at physiological pH (7 to 8) as opposed to more acidic environments. Expression of Pneumocystis CBK1 was also induced by contact with lung epithelial cells and extracellular matrix. Translation of this gene revealed extensive homology to other fungal CBK1 kinases. Pneumocystis CBK1 expression was equal in the cyst and trophic life forms of the organisms. We further demonstrate that Pneumocystis CBK1 expressed in cbk1 Delta Saccharomyces cerevisiae cells restored defective cell wall separation during proliferation. Consistent with this, Pneumocystis CBK1 expression also stimulated transcription of the CTS1 chitinase in cbk1 Delta mutant yeast cells, an event necessary for cell wall separation. In addition, Pneumocystis CBK1 cDNA supported normal mating projection formation in response to alpha-factor in the cbk1 Delta yeast cells. Site-directed mutations of serine-303 and threonine-494, potential regulatory phosphorylation sites in Pneumocystis CBK1, abolished mating projection formation, indicating a role for these amino acid residues in CBK1 activity. These findings indicate that Pneumocystis CBK1 is an environmentally responsive gene that may function in signaling pathways necessary for cell growth and mating.
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