ArticleInfection and immunity2004
Uropathogenic Escherichia coli triggers oxygen-dependent apoptosis in human neutrophils through the cooperative effect of type 1 fimbriae and lipopolysaccharide.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- D-mannose vs other agents for recurrent urinary tract infection prevention in adult women: a systematic review and meta-analysis.American journal of obstetrics and gynecology · 2020Pooled it
- Testosterone increases the virulence traits of uropathogenicFrontiers in microbiology · 2024Article
- Mechanisms of Keap1/Nrf2 modulation in bacterial infections: implications in persistence and clearance.Frontiers in immunology · 2024Review
- Uropathogen and host responses in pyelonephritis.Nature reviews. Nephrology · 2023Review
- Studies on Virulence and Extended-SpectrumBioMed research international · 2022Article
- Neutrophils: Many Ways to Die.Frontiers in immunology · 2021Review
- Neutrophil activation by Escherichia coli isolates from human intestine: effects of bacterial hydroperoxidase activity and surface hydrophobicity.FEBS open bio · 2020Article
- Magnolol and Honokiol Attenuate Apoptosis of Enterotoxigenic Escherichia Coli-Induced Intestinal Epithelium by Maintaining Secretion and Absorption Homeostasis and Protecting Mucosal Integrity.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Cell death-based approaches in treatment of the urinary tract-associated diseases: a fight for survival in the killing fields.Cell death & disease · 2018Review
- Mcl-1 is vital for neutrophil survival.Immunologic research · 2015Review
- Cellular immunity survey against urinary tract infection using pVAX/fimH cassette with mammalian and wild type codon usage as a DNA vaccine.Clinical and experimental vaccine research · 2014Article
- Mechanisms regulating neutrophil survival and cell death.Seminars in immunopathology · 2013Review
- Ubiquitin-proteasome-rich cytoplasmic structures in neutrophils of patients with Shwachman-Diamond syndrome.Haematologica · 2012Article
- Common genetic variations in the NALP3 inflammasome are associated with delayed apoptosis of human neutrophils.PloS one · 2012Article
- Protective role of Akt2 in Salmonella enterica serovar typhimurium-induced gastroenterocolitis.Infection and immunity · 2011Article
- Attenuation of human neutrophil migration and function by uropathogenic bacteria.Microbes and infection · 2011Article
- Afa/Dr-expressing, diffusely adhering Escherichia coli strain C1845 triggers F1845 fimbria-dependent phosphatidylserine externalization on neutrophil-like differentiated PLB-985 cells through an apoptosis-independent mechanism.Infection and immunity · 2010Article
- Enterotoxigenic Escherichia coli modulates host intestinal cell membrane asymmetry and metabolic activity.Infection and immunity · 2009Article
- Staying alive: bacterial inhibition of apoptosis during infection.Trends in microbiology · 2008Review
- Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis.Clinical microbiology reviews · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type 1 fimbriae are the most commonly expressed virulence factor on uropathogenic Escherichia coli. In addition to promoting avid bacterial adherence to the uroepithelium and enabling colonization, type 1 fimbriae recruit neutrophils to the urinary tract as an early inflammatory response. Using clinical isolates of type 1 fimbriated E. coli and an isogenic type 1 fimbria-negative mutant (CN1016) lacking the FimH adhesin, we investigated if these strains could modulate apoptosis in human neutrophils. We found that E. coli expressing type 1 fimbriae interacted with neutrophils in a mannose- and lipopolysaccharide (LPS)-dependent manner, leading to apoptosis which was triggered by the intracellular generation of reactive oxygen species. This induced neutrophil apoptosis was abolished by blocking FimH-mediated attachment, by inhibiting NADPH oxidase activation, or by neutralizing LPS. In contrast, CN1016, which did not adhere to or activate the respiratory burst of neutrophils, delayed the spontaneous apoptosis in an LPS-dependent manner. This delayed apoptosis could be mimicked by adding purified LPS and was also observed by using fimbriated bacteria in the presence of d-mannose. These results suggest that LPS is required for E. coli to exert both pro- and antiapoptotic effects on neutrophils and that the difference in LPS presentation (i.e., with or without fimbriae) determines the outcome. The present study showed that there is a fine-tuned balance between type 1 fimbria-induced and LPS-mediated delay of apoptosis in human neutrophils, in which altered fimbrial expression on uropathogenic E. coli determines the neutrophil survival and the subsequent inflammation during urinary tract infections.
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