ArticleThe Journal of cell biology1992
Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 84 citations in OpenAlex.
- Review
- Article
- Laminopathies: what can humans learn from fruit flies.Cellular & molecular biology letters · 2018Review
- Invertebrate models of lamin diseases.Nucleus (Austin, Tex.) · 2018Review
- Phosphoinositide 3-kinase beta protects nuclear envelope integrity by controlling RCC1 localization and Ran activity.Molecular and cellular biology · 2015Article
- Nuclear structure and chromosome segregation in Drosophila male meiosis depend on the ubiquitin ligase dTopors.Genetics · 2011Article
- Nuclear lamins: key regulators of nuclear structure and activities.Journal of cellular and molecular medicine · 2009Review
- A role for nuclear lamins in nuclear envelope assembly.The Journal of cell biology · 2001Article
- Nuclear lamins A and B1: different pathways of assembly during nuclear envelope formation in living cells.The Journal of cell biology · 2000Article
- The tail domain of lamin Dm0 binds histones H2A and H2B.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.The Journal of cell biology · 1999Article
- Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA.Molecular and cellular biology · 1998Article
- Nuclear membrane vesicle targeting to chromatin in a Drosophila embryo cell-free system.Molecular biology of the cell · 1997Article
- Localization and posttranslational modifications of otefin, a protein required for vesicle attachment to chromatin, during Drosophila melanogaster development.Molecular and cellular biology · 1997Article
- Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis.The Journal of cell biology · 1997Article
- Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae.The Journal of cell biology · 1997Article
- The lamin B receptor (LBR) provides essential chromatin docking sites at the nuclear envelope.The EMBO journal · 1996Article
- Targeting of membranes to sea urchin sperm chromatin is mediated by a lamin B receptor-like integral membrane protein.The Journal of cell biology · 1996Article
- The MAN antigens are non-lamin constituents of the nuclear lamina in vertebrate cells.Chromosoma · 1996Article
- Determinants for intracellular sorting of cytoplasmic and nuclear intermediate filaments.The Journal of cell biology · 1994Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of the Drosophila lamin protein in nuclear envelope assembly was studied using a Drosophila in vitro assembly system that reconstitutes nuclei from added sperm chromatin or naked DNA. Upon incubation of the embryonic assembly extract with anti-Drosophila lamin antibodies, the attachment of nuclear membrane vesicles to chromatin surface and nuclear envelope formation did not occur. Lamina assembly and nuclear membrane vesicles attachment to the chromatin were inhibited only when the activity of the 75-kD lamin isoform was inhibited in both soluble and membrane-vesicles fractions. Incubation of decondensed sperm chromatin with an extract that was depleted of nuclear membranes revealed the presence of lamin molecules on the chromatin periphery. In addition, high concentrations of bacterially expressed lamin molecules added to the extract, were able to associate with the chromatin periphery, and did not inhibit nuclear envelope assembly. After nuclear reconstitution, a fraction of the lamin pool was converted into the typical 74- and 76-kD isoforms. Together, these data strongly support an essential role of the lamina in nuclear envelope assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.