ArticlePlant physiology2004
Activity profiling of papain-like cysteine proteases in plants.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 156 citations in OpenAlex.
- The afterlife of a horizontally transferred gene: Expansion and functional diversification of a C1A peptidase in wild Hordeum species.The plant genome · 2026Article
- CitPH4 Confers Resistance to Citrus Canker by Activating Papain-Like Cysteine Protease.Plant biotechnology journal · 2026Article
- The rice cysteine protease OsEP3A promotes seedling growth and seed development and contains a nitrogen-starvation-responsive sequence.Plant cell reports · 2026Article
- Four guidelines to identify bona fide protease-substrate pairs.Journal of experimental botany · 2025Review
- Aphid effectors suppress plant immunity via recruiting defense proteins to processing bodies.Science advances · 2025Article
- Identification of a Papain-like Cysteine Protease Functioning as an Avirulence Factor in Striga-Cowpea Interactions.Plants (Basel, Switzerland) · 2025Article
- NbPIRIN promotes the protease activity of papain-like cysteine protease NbRD21 to inhibit Chinese wheat mosaic virus infection.PLoS pathogens · 2025Article
- Cathepsin B- and L-like Protease Activities Are Induced During Developmental Barley Leaf Senescence.Plants (Basel, Switzerland) · 2024Article
- Targeting Magnaporthe oryzae effector MoErs1 and host papain-like protease OsRD21 interaction to combat rice blast.Nature plants · 2024Article
- NIa-Pro of sugarcane mosaic virus targets Corn Cysteine Protease 1 (CCP1) to undermine salicylic acid-mediated defense in maize.PLoS pathogens · 2024Article
- The root pathogenFrontiers in plant science · 2023Article
- Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme.The New phytologist · 2022Article
- Knockout of a papain-like cysteine protease geneFrontiers in plant science · 2022Article
- Genome-wide association analysis of anthracnose resistance in sorghum [Sorghum bicolor (L.) Moench].PloS one · 2021Article
- Citrus Vascular Proteomics Highlights the Role of Peroxidases and Serine Proteases during Huanglongbing Disease Progression.Molecular & cellular proteomics : MCP · 2020Article
- Functional Omics Identifies Serine Hydrolases That Mobilize Storage Lipids during Rice Seed Germination.Plant physiology · 2020Article
- Barley cysteine protease PAP14 plays a role in degradation of chloroplast proteins.Journal of experimental botany · 2019Article
- Integrated Genome-Scale Analysis Identifies Novel Genes and Networks Underlying Senescence in Maize.The Plant cell · 2019Article
- Cutting Out the Gaps Between Proteases and Programmed Cell Death.Frontiers in plant science · 2019Article
- Proteases Underground: Analysis of the Maize Root Apoplast Identifies Organ Specific Papain-Like Cysteine Protease Activity.Frontiers in plant science · 2019Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptomic and proteomic technologies are generating a wealth of data that are frequently used by scientists to predict the function of proteins based on their expression or presence. However, activity of many proteins, such as transcription factors, kinases, and proteases, depends on posttranslational modifications that frequently are not detected by these technologies. Therefore, to monitor activity of proteases rather than their abundance, we introduce protease activity profiling in plants. This technology is based on the use of biotinylated, irreversible protease inhibitors that react with active proteases in a mechanism-based manner. Using a biotinylated derivative of the Cys protease inhibitor E-64, we display simultaneous activities of many papain-like Cys proteases in extracts from various tissues and from different plant species. Labeling is pH dependent, stimulated with reducing agents, and inhibited specifically by Cys protease inhibitors but not by inhibitors of other protease classes. Using one-step affinity capture of biotinylated proteases followed by sequencing mass spectrometry, we identified proteases that include xylem-specific XCP2, desiccation-induced RD21, and cathepsin B- and aleurain-like proteases. Together, these results demonstrate that this technology can identify differentially activated proteases and/or characterize the activity of a particular protease within complex mixtures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.