ArticleBritish journal of pharmacology2004
Matrix metalloproteinase expression and activity in human airway smooth muscle cells.
Article in British journal of pharmacology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00989742 (A Randomised, Double Blind, Placebo Controlled Trial of Doxycycline in Lymphangioleiomyomatosis.), which is not on this map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Randomised, Double Blind, Placebo Controlled Trial of Doxycycline in Lymphangioleiomyomatosis.
Who cites it
21 citing papers in PubMed, 74 citations in OpenAlex.
- Airway smooth muscle in asthma: insights from a transcriptomic meta-analysis.ERJ open research · 2026Article
- Airway remodeling in chronic obstructive pulmonary disease: characteristics and opportunities.Frontiers in medicine · 2025Review
- Functional role of glial-derived neurotrophic factor in a mixed allergen murine model of asthma.American journal of physiology. Lung cellular and molecular physiology · 2024Article
- Holy Grail: the journey towards disease modification in asthma.European respiratory review : an official journal of the European Respiratory Society · 2022Review
- The TL1A-DR3 Axis in Asthma: Membrane-Bound and Secreted TL1A Co-Determined the Development of Airway Remodeling.Allergy, asthma & immunology research · 2022Article
- Structure and function of small airways in asthma patients revisited.European respiratory review : an official journal of the European Respiratory Society · 2021Review
- Differential estrogen-receptor activation regulates extracellular matrix deposition in human airway smooth muscle remodelingFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Smooth muscle brain-derived neurotrophic factor contributes to airway hyperreactivity in a mouse model of allergic asthma.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway.Molecular medicine reports · 2018Article
- Brain-derived neurotrophic factor and airway fibrosis in asthma.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Monitoring inflammation and airway remodeling by fluorescence molecular tomography in a chronic asthma model.Journal of translational medicine · 2015Article
- CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- A fungal protease allergen provokes airway hyper-responsiveness in asthma.Nature communications · 2015Article
- Article
- Development and characterization of a 3D multicell microtissue culture model of airway smooth muscle.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Does airway smooth muscle express an inflammatory phenotype in asthma?British journal of pharmacology · 2011Review
- The pivotal role of airway smooth muscle in asthma pathophysiology.Journal of allergy · 2011Article
- IL-1beta induces expression of matrix metalloproteinase-9 and cell migration via a c-Src-dependent, growth factor receptor transactivation in A549 cells.British journal of pharmacology · 2010Article
- Airway smooth muscle in bronchial tone, inflammation, and remodeling: basic knowledge to clinical relevance.American journal of respiratory and critical care medicine · 2008Article
- Induction and regulation of matrix metalloproteinase-12 in human airway smooth muscle cells.Respiratory research · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Airway remodelling is a feature of chronic asthma comprising smooth muscle hypertrophy and deposition of extracellular matrix (ECM) proteins. Matrix metalloproteinases (MMPs) breakdown ECM, are involved in tissue remodelling and have been implicated in airway remodelling. Although mesenchymal cells are an important source of MMPs, little data are available on airway smooth muscle (ASM) derived MMPs. We therefore investigated MMP and tissue inhibitor of metalloproteinase (TIMP) production and activity in human ASM cells. MMPs and TIMPs were examined using quantitative real-time RT-PCR, Western blotting, zymography and a quench fluorescence (QF) assay of total MMP activity. The most abundant MMPs were pro-MMP-2, pro- MMP-3, active MMP-3 and MT1-MMP. TIMP-1 and TIMP-2 expression was low in cell lysates but high in conditioned medium. High TIMP secretion was confirmed by the ability of ASM-conditioned medium to inhibit recombinant MMP-2 in a QF assay. Thrombin increased MMP activity by activation of pro-MMP-2 independent of the conventional smooth muscle thrombin receptors PAR 1 and 4. In conclusion, ASM cells express pro-MMP-2, pro and active MMP-3, MMP-9 and MT1-MMP. Unstimulated cells secrete excess TIMP 1 and 2, preventing proteolytic activity. MMP-2 can be activated by thrombin which may contribute to airway remodelling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.