ArticleThe Biochemical journal2004
A newly identified zona pellucida glycoprotein, ZPD, and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 92 citations in OpenAlex.
- AlphaFold as a prior: experimental structure determination conditioned on a pretrained neural network.Nature methods · 2026Article
- Molecular weight-based profiling of sperm proteins and their association with semen quality in Kokok Balenggek roosters.Journal of advanced veterinary and animal research · 2025Article
- Expression, Localization, and Processing of Chicken Sperm ADAM32L2 during the Acrosome Reaction: A Possible Function in the Sperm-Egg Interaction.The journal of poultry science · 2025Article
- Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens (Gallus gallus).Journal of animal science and biotechnology · 2024Article
- Article
- Prediction of the Effect of Methylation in the Promoter Region of ZP2 Gene on Egg Production in Jinghai Yellow Chickens.Veterinary sciences · 2022Article
- Quantitative Protein Analysis of ZPB2, ZPB1 and ZPC in the Germinal Disc and a Non-Germinal Disc Region of the Inner Perivitelline Layer in Two Genetic Lines of Turkey Hens That Differ in Fertility.Animals : an open access journal from MDPI · 2022Article
- Transcriptome and Proteome Analysis Revealed Key Pathways Regulating Final Stage of Oocyte Maturation of the Turkey (International journal of molecular sciences · 2021Article
- Artificial selection reveals the role of transcriptional constraints in the maintenance of life history variation.Evolution letters · 2020Article
- Article
- Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility.Nature communications · 2019Article
- Dynamics of Structural Barriers and Innate Immune Components during Incubation of the Avian Egg: Critical Interplay between Autonomous Embryonic Development and Maternal Anticipation.Journal of innate immunity · 2019Review
- Bioinformatic analyses of zona pellucida genes in vertebrates and their expression in Nile tilapia.Fish physiology and biochemistry · 2018Article
- Egg Coat Proteins Across Metazoan Evolution.Current topics in developmental biology · 2018Review
- Sperm-Egg Interaction during Fertilization in Birds.The journal of poultry science · 2016Review
- A structured interdomain linker directs self-polymerization of human uromodulin.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Identification of distinctive interdomain interactions among ZP-N, ZP-C and other domains of zona pellucida glycoproteins underlying association of chicken egg-coat matrix.FEBS open bio · 2015Article
- Generation of transgenic rice with reduced content of major and novel high molecular weight allergens.Rice (New York, N.Y.) · 2014Article
- Evolution of genes involved in gamete interaction: evidence for positive selection, duplications and losses in vertebrates.PloS one · 2012Article
- Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors.Transgenic research · 2011Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fertilization begins with interaction between the sperm and the egg. The surface of the vertebrate oocyte is covered with the egg envelope, which is composed of ZP (zona pellucida) glycoproteins. We have identified two glycoproteins, ZP1/gp97 and ZPC/gp42, as the major components of the chicken egg envelope. In the present study, another 42 kDa protein, designated ZPD, has been found as a new major component of the chicken egg envelope. ZPD was specifically released from the egg envelope by ultrasonication treatment without urea. ZPD cDNA was cloned using a chicken granulosa cell cDNA pool. The deduced amino acid sequence showed that preproprotein of ZPD is composed of 418 amino acid residues with four potential N-glycosylation sites and includes a ZP domain, common in vertebrate ZP glycoproteins, and a transmembrane domain. ZPD belongs phylogenetically to a distinct group from known ZP glycoprotein subfamilies, ZPA, ZPB, and ZPC. In two-dimensional gel electrophoresis ZPD proteins were identified to be several isoforms with different pI values between 5 and 7. ZP1, ZPC and the newly identified ZPD were confirmed to be the major components of chicken egg envelope by MS of proteolytic digests of whole egg envelope. The in vitro incubation of chicken sperm with calcium ionophore A23187 induced sperm activation, resulting in the fragmentation and release of a 41 kDa PNA (peanut agglutinin)-positive glycoprotein and the decrease or loss of sperm PNA-stainability. The incubation with ZPD and dimeric ZP1, but not ZPC and monomeric ZP1, also induced the decrease or loss of sperm PNA-stainability, suggesting the in vitro sperm activation by these ZP components. Collectively, ZPD might bind loosely to egg envelope matrix and play a key role in the sperm activation on avian sperm-egg interaction.
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