ArticleJournal of structural and functional genomics2004
Automation of protein purification for structural genomics.
Article in Journal of structural and functional genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 105 citations in OpenAlex.
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- 3D domain swapping in the TIM barrel of the α subunit of Streptococcus pneumoniae tryptophan synthase.Acta crystallographica. Section D, Structural biology · 2020Article
- Structural analysis of free and liganded forms of the Fab fragment of a high-affinity anti-cocaine antibody, h2E2.Acta crystallographica. Section F, Structural biology communications · 2019Article
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- Functional Profiling and Crystal Structures of Isothiocyanate Hydrolases Found in Gut-Associated and Plant-Pathogenic Bacteria.Applied and environmental microbiology · 2018Article
- Crystal Structure of Thioesterase SgcE10 Supporting Common Polyene Intermediates in 9- and 10-Membered Enediyne Core Biosynthesis.ACS omega · 2017Article
- The CDI toxin of Yersinia kristensenii is a novel bacterial member of the RNase A superfamily.Nucleic acids research · 2017Article
- A novel signal transduction protein: Combination of solute binding and tandem PAS-like sensor domains in one polypeptide chain.Protein science : a publication of the Protein Society · 2017Article
- Crystal structure of SgcJ, an NTF2-like superfamily protein involved in biosynthesis of the nine-membered enediyne antitumor antibiotic C-1027.The Journal of antibiotics · 2016Article
- A microbial sensor for organophosphate hydrolysis exploiting an engineered specificity switch in a transcription factor.Nucleic acids research · 2016Article
- How Aromatic Compounds Block DNA Binding of HcaR Catabolite Regulator.The Journal of biological chemistry · 2016Article
- Co-occurrence of analogous enzymes determines evolution of a novel (βα)8-isomerase sub-family after non-conserved mutations in flexible loop.The Biochemical journal · 2016Article
- A novel transcriptional regulator of L-arabinose utilization in human gut bacteria.Nucleic acids research · 2015Article
- EsxB, a secreted protein from Bacillus anthracis forms two distinct helical bundles.Protein science : a publication of the Protein Society · 2015Article
- New aminopeptidase from "microbial dark matter" archaeon.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Hydrogen bond donation to the heme distal ligand of Staphylococcus aureus IsdG tunes the electronic structure.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2015Article
- Biochemical and structural analysis of an Eis family aminoglycoside acetyltransferase from bacillus anthracis.Biochemistry · 2015Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
A critical issue in structural genomics, and in structural biology in general, is the availability of high-quality samples. The additional challenge in structural genomics is the need to produce high numbers of proteins with low sequence similarities and poorly characterized or unknown properties. 'Structural-biology-grade' proteins must be generated in a quantity and quality suitable for structure determination experiments using X-ray crystallography or nuclear magnetic resonance (NMR). The choice of protein purification and handling procedures plays a critical role in obtaining high-quality protein samples. The purification procedure must yield a homogeneous protein and must be highly reproducible in order to supply milligram quantities of protein and/or its derivative containing marker atom(s). At the Midwest Center for Structural Genomics we have developed protocols for high-throughput protein purification. These protocols have been implemented on AKTA EXPLORER 3D and AKTA FPLC 3D workstations capable of performing multidimensional chromatography. The automated chromatography has been successfully applied to many soluble proteins of microbial origin. Various MCSG purification strategies, their implementation, and their success rates are discussed in this paper.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.