ArticleJournal of structural and functional genomics2004
Production of selenomethionine-labeled proteins in two-liter plastic bottles for structure determination.
Article in Journal of structural and functional genomics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
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- Biochemical and structural studies of two tetrameric nucleoside 2'-deoxyribosyltransferases from psychrophilic and mesophilic bacteria: Insights into cold-adaptation.International journal of biological macromolecules · 2021Article
- Structures of teixobactin-producing nonribosomal peptide synthetase condensation and adenylation domains.Current research in structural biology · 2020Article
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- Structural basis of ECF-σ-factor-dependent transcription initiation.Nature communications · 2019Article
- Natural separation of the acyl-CoA ligase reaction results in a non-adenylating enzyme.Nature chemical biology · 2018Article
- Structure of a novel antibacterial toxin that exploits elongation factor Tu to cleave specific transfer RNAs.Nucleic acids research · 2017Article
- Structural Basis of Mycobacterium tuberculosis Transcription and Transcription Inhibition.Molecular cell · 2017Article
- Current strategies for protein production and purification enabling membrane protein structural biology.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- Structure of the ent-Copalyl Diphosphate Synthase PtmT2 from Streptomyces platensis CB00739, a Bacterial Type II Diterpene Synthase.Journal of the American Chemical Society · 2016Article
- New aminopeptidase from "microbial dark matter" archaeon.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Subdomain II of α-isopropylmalate synthase is essential for activity: inferring a mechanism of feedback inhibition.The Journal of biological chemistry · 2014Article
- RsaM: a transcriptional regulator of Burkholderia spp. with novel fold.The FEBS journal · 2014Article
- Protein production for structural genomics using E. coli expression.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Structure of the St. Louis encephalitis virus postfusion envelope trimer.Journal of virology · 2013Article
- High-throughput protein purification and quality assessment for crystallization.Methods (San Diego, Calif.) · 2011Review
- SAD phasing using iodide ions in a high-throughput structural genomics environment.Journal of structural and functional genomics · 2011Article
- Cleavable C-terminal His-tag vectors for structure determination.Journal of structural and functional genomics · 2010Article
- Crystallization and preliminary X-ray diffraction studies of FAD synthetase from Corynebacterium ammoniagenes.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A simplified approach developed recently for the production of heterologous proteins in Escherichia coli uses 2-liter polyethylene terephthalate beverage bottles as disposable culture vessels [Sanville Millard, C. et al. 2003. Protein Expr. Purif. 29, 311-320]. The method greatly reduces the time and effort needed to produce native proteins for structural or functional studies. We now demonstrate that the approach is also well suited for production of proteins in defined media with incorporation of selenomethionine to facilitate structure determination by multiwavelength anomalous diffraction. Induction of a random set of Bacillus stearothermophilus target genes under the new protocols generated soluble selenomethionyl proteins in good yield. Several selenomethionyl proteins were purified in good yields and three were subjected to amino acid analysis. Incorporation of selenomethionine was determined to be greater than 95% in one protein and greater than 98% in the other two. In the preceding paper [Zhao et al., this issue, pp. 87-93], the approach is further extended to production of [U-15N]- or [U-13C, U-15N]-labeled proteins. The approach thus appears suitable for high-throughput production of proteins for structure determination by X-ray crystallography or nuclear magnetic resonance spectroscopy.
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Registered trials
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