ArticleThe Journal of cell biology2004
Novel signaling pathways mediating reciprocal control of keratinocyte migration and wound epithelialization through M3 and M4 muscarinic receptors.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- The Muscarinic Acetylcholine Receptor in Dermal Papilla Cells Regulates Hair Growth.International journal of molecular sciences · 2026Article
- The Role of Muscarinic Acetylcholine Receptor MInternational journal of molecular sciences · 2024Review
- Article
- A growing understanding of the role of muscarinic receptors in the molecular pathology and treatment of schizophrenia.Frontiers in cellular neuroscience · 2023Review
- The M3 Muscarinic Acetylcholine Receptor Promotes Epidermal Differentiation.The Journal of investigative dermatology · 2022Article
- The Modulatory Influence of Plant-Derived Compounds on Human Keratinocyte Function.International journal of molecular sciences · 2021Review
- Multiple Roles for Cholinergic Signaling from the Perspective of Stem Cell Function.International journal of molecular sciences · 2021Review
- Extrasynaptic acetylcholine signaling through a muscarinic receptor regulates cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model.Developmental cell · 2020Article
- Biological behaviors of muscarinic receptors in mesenchymal stem cells derived from human placenta and bone marrow.Iranian journal of basic medical sciences · 2020Article
- Tissue-scale coordination of cellular behaviour promotes epidermal wound repair in live mice.Nature cell biology · 2017Article
- The Roles of Growth Factors in Keratinocyte Migration.Advances in wound care · 2015Review
- Epidermal growth factor receptor transactivation is required for mitogen-activated protein kinase activation by muscarinic acetylcholine receptors in HaCaT keratinocytes.International journal of molecular sciences · 2014Article
- The acetylcholine signaling network of corneal epithelium and its role in regulation of random and directional migration of corneal epithelial cells.Investigative ophthalmology & visual science · 2014Article
- Article
- Acetylcholine induces mesenchymal stem cell migration via Ca2+ /PKC/ERK1/2 signal pathway.Journal of cellular biochemistry · 2012Article
- Auto/paracrine nicotinergic peptides participate in cutaneous stress response to wounding.Dermato-endocrinology · 2012Article
- NHE1 activity contributes to migration and is necessary for proliferation of human gastric myofibroblasts.Pflugers Archiv : European journal of physiology · 2012Article
- Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system.Advances in anatomy, embryology, and cell biology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
To test the hypothesis that keratinocyte (KC) migration is modulated by distinct muscarinic acetylcholine (ACh) receptor subtypes, we inactivated signaling through specific receptors in in vitro and in vivo models of reepithelialization by subtype-selective antagonists, small interfering RNA, and gene knockout in mice. KC migration and wound reepithelialization were facilitated by M4 and inhibited by M3. Additional studies showed that M4 increases expression of "migratory" integrins alpha5beta1, alphaVbeta5, and alphaVbeta6, whereas M3 up-regulates "sedentary" integrins alpha2beta1 and alpha3beta1. Inhibition of migration by M3 was mediated through Ca2+-dependent guanylyl cyclase-cyclic GMP-protein kinase G signaling pathway. The M4 effects resulted from inhibition of the inhibitory pathway involving the adenylyl cyclase-cyclic AMP-protein kinase A pathway. Both signaling pathways intersected at Rho, indicating that Rho kinase provides a common effector for M3 and M4 regulation of cell migration. These findings offer novel insights into the mechanisms of ACh-mediated modulation of KC migration and wound reepithelialization, and may aid the development of novel methods to promote wound healing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.