ArticleJournal of bacteriology2004
Three-dimensional electron microscopic imaging of membrane invaginations in Escherichia coli overproducing the chemotaxis receptor Tsr.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Mechanisms of E. coli chemotaxis signaling pathways visualized using cryoET and computational approaches.Biochemical Society transactions · 2022Review
- Inducible intracellular membranes: molecular aspects and emerging applications.Microbial cell factories · 2020Review
- Structural and Proteomic Changes in Viable but Non-culturableFrontiers in microbiology · 2019Article
- Ectopic Neo-Formed Intracellular Membranes inBiomolecules · 2018Review
- His-Tag-Mediated Dimerization of Chemoreceptors Leads to Assembly of Functional Nanoarrays.Biochemistry · 2017Article
- Origins of chemoreceptor curvature sorting in Escherichia coli.Nature communications · 2017Article
- A Complex Endomembrane System in the ArchaeonFrontiers in microbiology · 2017Article
- New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography.Biochemistry · 2014Article
- Regulation of lipid biosynthesis, sliding motility, and biofilm formation by a membrane-anchored nucleoid-associated protein of Mycobacterium tuberculosis.Journal of bacteriology · 2013Article
- Self-association of the histidine kinase CheA as studied by pulsed dipolar ESR spectroscopy.Biophysical journal · 2012Article
- Chemoreceptors and flagellar motors are subterminally located in close proximity at the two cell poles in spirochetes.Journal of bacteriology · 2011Article
- Protein-induced modulation of chloroplast membrane morphology.Frontiers in plant science · 2011Article
- Kinase-active signaling complexes of bacterial chemoreceptors do not contain proposed receptor-receptor contacts observed in crystal structures.Biochemistry · 2010Article
- Universal architecture of bacterial chemoreceptor arrays.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Cryo-electron tomography of bacteria: progress, challenges and future prospects.Nature reviews. Microbiology · 2009Review
- The core signaling proteins of bacterial chemotaxis assemble to form an ultrastable complex.Biochemistry · 2009Article
- Polar chemoreceptor clustering by coupled trimers of dimers.Biophysical journal · 2009Article
- Role of HAMP domains in chemotaxis signaling by bacterial chemoreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Location and architecture of the Caulobacter crescentus chemoreceptor array.Molecular microbiology · 2008Article
- Ultrastructural characterization of the prokaryotic symbiosis in "Chlorochromatium aggregatum".Journal of bacteriology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Electron tomography is a powerful method for determining the three-dimensional structures of large macromolecular assemblies, such as cells, organelles, and multiprotein complexes, when crystallographic averaging methods are not applicable. Here we used electron tomographic imaging to determine the molecular architecture of Escherichia coli cells engineered to overproduce the bacterial chemotaxis receptor Tsr. Tomograms constructed from fixed, cryosectioned cells revealed that overproduction of Tsr led to formation of an extended internal membrane network composed of stacks and extended tubular structures. We present an interpretation of the tomogram in terms of the packing arrangement of Tsr using constraints derived from previous X-ray and electron-crystallographic studies of receptor clusters. Our results imply that the interaction between the cytoplasmic ends of Tsr is likely to stabilize the presence of the membrane networks in cells overproducing Tsr. We propose that membrane invaginations that are potentially capable of supporting axial interactions between receptor clusters in apposing membranes could also be present in wild-type E. coli and that such receptor aggregates could play an important role in signal transduction during bacterial chemotaxis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.