ArticleJournal of bacteriology2004
Identification of the protease and the turnover signal responsible for cell cycle-dependent degradation of the Caulobacter FliF motor protein.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Thioredoxin and its partner protein are essential for zoospore flagellar formation in Actinoplanes missouriensis.Communications biology · 2026Article
- Azospirillum brasilense AerC and Tlp4b Cytoplasmic Chemoreceptors Are Promiscuous and Interact with the Two Membrane-Bound Chemotaxis Signaling Clusters Mediating Chemotaxis Responses.Journal of bacteriology · 2023Article
- Proteolysis dependent cell cycle regulation in Caulobacter crescentus.Cell division · 2022Review
- The Protein Quality Control Network inFrontiers in molecular biosciences · 2021Review
- Construction and Loss of Bacterial Flagellar Filaments.Biomolecules · 2020Review
- Programmed Proteolysis of Chemotaxis Proteins in Sinorhizobium meliloti: Features in the C-Terminal Region Control McpU Degradation.Journal of bacteriology · 2020Article
- In situ imaging of the bacterial flagellar motor disassembly and assembly processes.The EMBO journal · 2019Article
- γ-proteobacteria eject their polar flagella under nutrient depletion, retaining flagellar motor relic structures.PLoS biology · 2019Article
- An Integrated Systems Approach Unveils New Aspects of Microoxia-Mediated Regulation inFrontiers in microbiology · 2019Article
- Hit the right spots: cell cycle control by phosphorylated guanosines in alphaproteobacteria.Nature reviews. Microbiology · 2017Review
- ClpAP is an auxiliary protease for DnaA degradation in Caulobacter crescentus.Molecular microbiology · 2016Article
- Regulated Proteolysis in Bacteria: Caulobacter.Annual review of genetics · 2016Review
- ClpXP and ClpAP proteolytic activity on divisome substrates is differentially regulated following the Caulobacter asymmetric cell division.Molecular microbiology · 2014Article
- The coding and noncoding architecture of the Caulobacter crescentus genome.PLoS genetics · 2014Article
- Regulated proteolysis in bacterial development.FEMS microbiology reviews · 2014Review
- Regulatory cohesion of cell cycle and cell differentiation through interlinked phosphorylation and second messenger networks.Molecular cell · 2011Article
- Complex regulatory pathways coordinate cell-cycle progression and development in Caulobacter crescentus.Advances in microbial physiology · 2009Review
- Get the message out: cyclic-Di-GMP regulates multiple levels of flagellum-based motility.Journal of bacteriology · 2008Review
- A phospho-signaling pathway controls the localization and activity of a protease complex critical for bacterial cell cycle progression.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flagellar ejection is tightly coupled to the cell cycle in Caulobacter crescentus. The MS ring protein FliF, which anchors the flagellar structure in the inner membrane, is degraded coincident with flagellar release. Previous work showed that removal of 26 amino acids from the C terminus of FliF prevents degradation of the protein and interferes with flagellar assembly. To understand FliF degradation in more detail, we identified the protease responsible for FliF degradation and performed a high-resolution mutational analysis of the C-terminal degradation signal of FliF. Cell cycle-dependent turnover of FliF requires an intact clpA gene, suggesting that the ClpAP protease is required for removal of the MS ring protein. Deletion analysis of the entire C-terminal cytoplasmic portion of FliF C confirmed that the degradation signal was contained in the last 26 amino acids that were identified previously. However, only deletions longer than 20 amino acids led to a stable FliF protein, while shorter deletions dispersed over the entire 26 amino acids critical for turnover had little effect on stability. This indicated that the nature of the degradation signal is not based on a distinct primary amino acid sequence. The addition of charged amino acids to the C-terminal end abolished cell cycle-dependent FliF degradation, implying that a hydrophobic tail feature is important for the degradation of FliF. Consistent with this, ClpA-dependent degradation was restored when a short stretch of hydrophobic amino acids was added to the C terminus of stable FliF mutant forms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.