ArticleWorld journal of gastroenterology2004
Hepatitis B virus X gene induces human telomerase reverse transcriptase mRNA expression in cultured normal human cholangiocytes.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Pathogenesis of Hepatocellular Carcinoma: The Interplay of Apoptosis and Autophagy.Biomedicines · 2023Review
- Review
- Identification of key genes in hepatitis B associated hepatocellular carcinoma based on WGCNA.Infectious agents and cancer · 2021Article
- Serum-reduced media impacts on cell viability and protein expression in human lung epithelial cells.Journal of cellular physiology · 2019Article
- Molecular Mechanisms Driving Progression of Liver Cirrhosis towards Hepatocellular Carcinoma in Chronic Hepatitis B and C Infections: A Review.International journal of molecular sciences · 2019Review
- TIP60 represses telomerase expression by inhibiting Sp1 binding to the TERT promoter.PLoS pathogens · 2017Article
- HBV-associated intrahepatic cholangiocarcinoma with high serum alpha-fetoprotein: a case report with review of literature.BMC infectious diseases · 2016Review
- A potential association between exposure to hepatitis B virus and small bowel adenocarcinoma.Journal of gastrointestinal oncology · 2016Article
- Cause-specific telomere factors deregulation in hepatocellular carcinoma.Journal of experimental & clinical cancer research : CR · 2013Article
- Risk factors for intrahepatic cholangiocarcinoma: a possible role of hepatitis B virus.Journal of viral hepatitis · 2010Article
- Risk factors for intrahepatic cholangiocarcinoma: a case-control study in China.World journal of gastroenterology · 2008Article
- Inhibitory effects of antisense phosphorothioate oligodeoxynucleotides on pancreatic cancer cell Bxpc-3 telomerase activity and cell growth in vitro.World journal of gastroenterology · 2006Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the transcriptional regulation of human telomerase reverse transcriptase (hTERT) mRNA in normal human cholangiocytes (HBECs) after hepatitis B virus X (HBx) gene transfection and to elucidate the possible mechanism of HBV infection underlying cholangiocarcinoma.
methodsHBECs were cultured in vitro and co-transfected with a eukaryotic expression vector containing the HBx coding region and a cloning vector containing coding sequences of enhanced green fluorescent protein (EGFP) using lipid-mediated gene transfer. The transfection efficiency was determined by the expression of EGFP. The expressions of hTERT mRNA and HBx protein in HBECs were detected by RT-PCR and immunocytochemical stain, respectively.
resultsThe transfection efficiencies were about 15% for both HBx gene expression plasmid and empty vector. No hTERT mRNA was expressed in HBECs when transfected with OPTI-MEM medium and empty vector, but a dramatic increase was observed for hTERT mRNA expression in HBECs when transfected with HBx expression vector. HBx protein was only expressed in HBECs when transfected with HBx expression vector.
conclusionHBx transfection can activate the transcriptional expression of hTERT mRNA. Cis-activation of hTERT mRNA by HBx gene is the primary mechanism underlying the proliferation, differentiation and tumorigenesis of biliary epithelia.
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