ArticleThe EMBO journal2004
PAM mediates sustained inhibition of cAMP signaling by sphingosine-1-phosphate.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- Understanding the role of GhRCC1.61 in flowering time in upland cotton through integrated analyses of gene family, RNA-seq, VIGS and allelic variation.Plant cell reports · 2025Article
- Sphingosine 1-phosphate Stimulates Insulin Secretion and Improves Cell Survival by Blocking Voltage-dependent KFrontiers in pharmacology · 2021Article
- Transcriptional Regulation of Genes by Ikaros Tumor Suppressor in Acute Lymphoblastic Leukemia.International journal of molecular sciences · 2020Review
- FBXO45-MYCBP2 regulates mitotic cell fate by targeting FBXW7 for degradation.Cell death and differentiation · 2020Article
- Phosphoproteomic analysis of the antitumor effects of ginsenoside Rg3 in human breast cancer cells.Oncology letters · 2018Article
- The complex of TRIP-Br1 and XIAP ubiquitinates and degrades multiple adenylyl cyclase isoforms.eLife · 2017Article
- Article
- The PHR proteins: intracellular signaling hubs in neuronal development and axon degeneration.Neural development · 2016Review
- RPM-1 uses both ubiquitin ligase and phosphatase-based mechanisms to regulate DLK-1 during neuronal development.PLoS genetics · 2014Article
- Identification of potential mediators of retinotopic mapping: a comparative proteomic analysis of optic nerve from WT and Phr1 retinal knockout mice.Journal of proteome research · 2012Article
- The E3 ubiquitin ligase protein associated with Myc (Pam) regulates mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling in vivo through N- and C-terminal domains.The Journal of biological chemistry · 2012Article
- Regulation of neuronal functions by the E3-ubiquitinligase protein associated with MYC (MYCBP2).Communicative & integrative biology · 2011Article
- Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons.Nature neuroscience · 2011Article
- The ubiquitin ligase MYCBP2 regulates transient receptor potential vanilloid receptor 1 (TRPV1) internalization through inhibition of p38 MAPK signaling.The Journal of biological chemistry · 2011Article
- Anti-inflammatory role of microsomal prostaglandin E synthase-1 in a model of neuroinflammation.The Journal of biological chemistry · 2011Article
- Gene expression profiling of oral squamous cell carcinoma by differential display rt-PCR and identification of tumor biomarkers.Indian journal of surgical oncology · 2010Article
- Characterization of zebrafish Esrom (Myc-binding protein 2) RCC1-like domain splice variants.Molecular and cellular biochemistry · 2010Article
- Structures of PHR domains from Mus musculus Phr1 (Mycbp2) explain the loss-of-function mutation (Gly1092-->Glu) of the C. elegans ortholog RPM-1.Journal of molecular biology · 2010Article
- Fbxo45 forms a novel ubiquitin ligase complex and is required for neuronal development.Molecular and cellular biology · 2009Article
- Phr1 regulates retinogeniculate targeting independent of activity and ephrin-A signalling.Molecular and cellular neurosciences · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PAM (Protein Associated with Myc) is an almost ubiquitously expressed protein that is one of the most potent inhibitors of adenylyl cyclase activity known so far. Here we show that PAM is localized at the endoplasmic reticulum in HeLa cells and that upon serum treatment PAM is recruited to the plasma membrane, causing an inhibition of adenylyl cyclase activity. We purified the serum factor that induced PAM translocation and identified it as sphingosine-1-phosphate (S1P). Within 15 min after incubation with S1P, PAM appeared at the plasma membrane and was detectable for up to 120 min. Sphingosine-1-phosphate induced adenylyl cyclase inhibition in two phases: an initial (1-10 min) and a late (20-240 min) phase. The initial adenylyl cyclase inhibition was Gi-mediated and PAM independent. In the late phase, adenylyl cyclase inhibition was PAM dependent and attenuated cyclic AMP (cAMP) signaling by various cAMP-elevating signals. This makes PAM the longest lasting nontranscriptional regulator of adenylyl cyclase activity known to date and presents a novel mechanism for the temporal regulation of cAMP signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.