ArticleGenes & development2004
Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 68 citations in OpenAlex.
- Autophagy-related protein Atg11 is essential for microtubule-mediated chromosome segregation.PLoS biology · 2025Article
- APC/CCdc20-mediated degradation of Clb4 prompts astral microtubule stabilization at anaphase onset.The Journal of cell biology · 2023Article
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- Article
- Asymmetric distribution of glucose transporter mRNA provides a growth advantage in yeast.The EMBO journal · 2019Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Molecular basis of Kar9-Bim1 complex function during mating and spindle positioning.Molecular biology of the cell · 2016Article
- Control of microtubule organization and dynamics: two ends in the limelight.Nature reviews. Molecular cell biology · 2015Review
- Coupling spindle position with mitotic exit in budding yeast: The multifaceted role of the small GTPase Tem1.Small GTPases · 2015Review
- G1 cyclin driven DNA replication.Cell cycle (Georgetown, Tex.) · 2015Article
- The yeast homologue of the microtubule-associated protein Lis1 interacts with the sumoylation machinery and a SUMO-targeted ubiquitin ligase.Molecular biology of the cell · 2012Article
- Purification, crystallization and preliminary crystallographic analysis of Kar9p from Saccharomyces cerevisiae.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- The MEN mediates the effects of the spindle assembly checkpoint on Kar9-dependent spindle pole body inheritance in budding yeast.Cell cycle (Georgetown, Tex.) · 2012Article
- Astral microtubule asymmetry provides directional cues for spindle positioning in budding yeast.Experimental cell research · 2012Review
- The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4.The Journal of cell biology · 2011Article
- Global analysis of Cdc14 phosphatase reveals diverse roles in mitotic processes.The Journal of biological chemistry · 2011Article
- Mutation of Ser172 in yeast β tubulin induces defects in microtubule dynamics and cell division.PloS one · 2010Article
- Candida albicans cyclin Clb4 carries S-phase cyclin activity.Eukaryotic cell · 2010Article
- Spindle position is coordinated with cell-cycle progression through establishment of mitotic exit-activating and -inhibitory zones.Molecular cell · 2010Article
- Actin-mediated delivery of astral microtubules instructs Kar9p asymmetric loading to the bud-ward spindle pole.Molecular biology of the cell · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, associates only with the SPB in the mother cell and so prevents Kar9 binding to this SPB. However, here we show that the nonoverexpressed Clb4 associates with the budward-directed SPB through Kar9. Cdc28-Clb4 then uses Kar9 as a carrier to move from this SPB to the plus ends of astral microtubules, where Cdc28-Clb4 regulates the interactions between microtubule ends and subdomains of the bud cortex. In the absence of Cdc28-Clb4 activity (G1/S phase), astral microtubules interact with the bud tip in a manner dependent on actin, Myo2, and Kar9. Coincidentally with reaching the bud cortex in S phase, Cdc28-Clb4 facilitates the dissociation of the microtubule bud tip interaction and their capture by the bud neck. This transition prevents the preanaphase spindle from becoming prematurely pulled into the bud. Thus, Cdc28-Clb4 facilitates spindle alignment by regulating the interaction of astral microtubules with subdomains of the bud cortex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.