ArticleInternational journal of experimental pathology2004
In situ analysis of lung antigen-presenting cells during murine pulmonary infection with virulent Mycobacterium tuberculosis.
Article in International journal of experimental pathology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Relatively low level of antigen-specific monocytes detected in blood from untreated tuberculosis patients using CD4+ T-cell receptor tetramers.PLoS pathogens · 2012Trial
- MARCO variants are associated with phagocytosis, pulmonary tuberculosis susceptibility and Beijing lineage.Genes and immunity · 2016Article
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- Genetic variants of MARCO are associated with susceptibility to pulmonary tuberculosis in a Gambian population.BMC medical genetics · 2013Article
- Spatiotemporally separated antigen uptake by alveolar dendritic cells and airway presentation to T cells in the lung.The Journal of experimental medicine · 2012Article
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- The tuberculous granuloma: an unsuccessful host defence mechanism providing a safety shelter for the bacteria?Clinical & developmental immunology · 2012Review
- Dispensability of surfactant proteins A and D in immune control of Mycobacterium tuberculosis infection following aerosol challenge of mice.Infection and immunity · 2011Article
- Kinetics and cellular sources of cathelicidin during the course of experimental latent tuberculous infection and progressive pulmonary tuberculosis.Clinical and experimental immunology · 2010Article
- Regulation of antigen presentation by Mycobacterium tuberculosis: a role for Toll-like receptors.Nature reviews. Microbiology · 2010Review
- MARCO, TLR2, and CD14 are required for macrophage cytokine responses to mycobacterial trehalose dimycolate and Mycobacterium tuberculosis.PLoS pathogens · 2009Article
- Mycobacterium bovis BCG decreases MHC-II expression in vivo on murine lung macrophages and dendritic cells during aerosol infection.Cellular immunology · 2009Article
- Distinct chemokine and cytokine gene expression pattern of murine dendritic cells and macrophages in response to Mycobacterium tuberculosis infection.Journal of leukocyte biology · 2008Article
- Early T-cell responses in tuberculosis immunity.Immunological reviews · 2008Review
- Respiratory dendritic cells: mediators of tolerance and immunity.Immunologic research · 2007Review
- In vivo role of dendritic cells in a murine model of pulmonary cryptococcosis.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Scarce information exists about the role of lung antigen-presenting cells (APCs) in vivo during pulmonary tuberculosis. As APCs activate cellular immunity, following intratracheal inoculation with virulent Mycobacterium tuberculosis, we assessed in situ lung APC recruitment, distribution, granuloma involvement, morphology and mycobacterial burden by using MHC-CII, CD14, scavenger receptor class A (SRA), the murine dendritic cell (DC)-restricted marker CD11c and Ziehl-Neelsen staining. CD11c(+) DC and CD14(+) cell recruitment into lungs appeared by day 14, continuing until day 60. MHC-CII(+) cells increased since day 7, persisting until day 60. Thus, virulent mycobacteria delays (14-21 days) lung APC recruitment compared to model antigens and nonvirulent bacilli (24-48 h). Regarding granuloma constitution, highly bacillary CD14(+) and SRA(+) cells were centrally located. MHC-CII(+) cells were more peripheral, with less mycobacteria. CD11c(+) cells were heterogeneously distributed within granulomas, with scarce bacilli. When labelling lung suspensions for MHC-CII and classifying cells as macrophages or DC, then staining for Ziehl-Neelsen, a remarkable segregation was found regarding bacillary burden. Most macrophage-like cells contained numerous bacilli, while DC had no or scarce mycobacteria. This implies differential APC contributions in situ during pulmonary tuberculosis regarding mycobacterial uptake, granuloma involvement and perhaps bacillary growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.