ArticleNucleic acids research2004
Inhibition of HIV-1 multiplication by antisense U7 snRNAs and siRNAs targeting cyclophilin A.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- U7 snRNA: A tool for gene therapy.The journal of gene medicine · 2021Review
- Pathological mechanism and antisense oligonucleotide-mediated rescue of a non-coding variant suppressing factor 9 RNA biogenesis leading to hemophilia B.PLoS genetics · 2020Article
- Antisense Oligonucleotides Promote Exon Inclusion and Correct the Common c.-32-13T>G GAA Splicing Variant in Pompe Disease.Molecular therapy. Nucleic acids · 2017Article
- Review
- Small-sized, stable lipid nanoparticle for the efficient delivery of siRNA to human immune cell lines.Scientific reports · 2016Article
- Characterization of the Anti-HCV Activities of the New Cyclophilin Inhibitor STG-175.PloS one · 2016Article
- A Multiple siRNA-Based Anti-HIV/SHIV Microbicide Shows Protection in Both In Vitro and In Vivo Models.PloS one · 2015Article
- Protection of shrimp Penaeus monodon from WSSV infection using antisense constructs.Marine biotechnology (New York, N.Y.) · 2014Article
- Antiviral Stratagems Against HIV-1 Using RNA Interference (RNAi) Technology.Evolutionary bioinformatics online · 2013Article
- An exon-specific U1 small nuclear RNA (snRNA) strategy to correct splicing defects.Human molecular genetics · 2012Article
- The isomerase active site of cyclophilin A is critical for hepatitis C virus replication.The Journal of biological chemistry · 2009Article
- Doxycycline-controlled splicing modulation by regulated antisense U7 snRNA expression cassettes.Gene therapy · 2009Article
- Alternative splicing and disease.Biochimica et biophysica acta · 2009Review
- Nucleic acids-based therapeutics in the battle against pathogenic viruses.Handbook of experimental pharmacology · 2009Review
- RNA interference against viruses: strike and counterstrike.Nature biotechnology · 2007Review
- Therapeutic potential of RNA interference against cellular targets of HIV infection.Molecular biotechnology · 2007Review
- Antisense-mediated exon skipping: a versatile tool with therapeutic and research applications.RNA (New York, N.Y.) · 2007Review
- Regulatory polymorphisms in the cyclophilin A gene, PPIA, accelerate progression to AIDS.PLoS pathogens · 2007Article
- Small interfering RNAs against the TAR RNA binding protein, TRBP, a Dicer cofactor, inhibit human immunodeficiency virus type 1 long terminal repeat expression and viral production.Journal of virology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human immunodeficiency virus 1 (HIV-1) multiplication depends on a cellular protein, cyclophilin A (CyPA), that gets integrated into viral particles. Because CyPA is not required for cell viability, we attempted to block its synthesis in order to inhibit HIV-1 replication. For this purpose, we used antisense U7 small nuclear RNAs (snRNAs) that disturb CyPA pre-mRNA splicing and short interfering RNAs (siRNAs) that target CyPA mRNA for degradation. With dual-specificity U7 snRNAs targeting the 3' and 5' splice sites of CyPA exons 3 or 4, we obtained an efficient skipping of these exons and a strong reduction of CyPA protein. Furthermore, short interfering RNAs targeting two segments of the CyPA coding region strongly reduced CyPA mRNA and protein levels. Upon lentiviral vector-mediated transduction, prolonged antisense effects were obtained for both types of antisense RNAs in the human T-cell line CEM-SS. These transduced CEM-SS cells showed a delayed, and for the siRNAs also reduced, HIV-1 multiplication. Since the two types of antisense RNAs function by different mechanisms, combining the two approaches may result in a synergistic effect.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.