ArticleMolecular biology of the cell2004
Cell context-specific effects of the beta-tubulin glycylation domain on assembly and size of microtubular organelles.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 62 citations in OpenAlex.
- Emerging Roles of Tubulin Isoforms and Their Post-Translational Modifications in Microtubule-Based Transport and Cellular Functions.Biomolecules · 2026Review
- The TTLL10 polyglycylase is stimulated by tubulin glutamylation and inhibited by polyglycylation.bioRxiv : the preprint server for biology · 2025Article
- Left-right cortical interactions drive intracellular pattern formation in the ciliate Tetrahymena.PLoS genetics · 2025Article
- Anterior-posterior pattern formation in ciliates.The Journal of eukaryotic microbiology · 2022Review
- Ciliate cortical organization and dynamics for cell motility: Comparing ciliates and vertebrates.The Journal of eukaryotic microbiology · 2022Review
- Tubulin posttranslational modifications modify the atypical spermatozoon centriole.microPublication biology · 2022Article
- Polarity in Ciliate Models: From Cilia to Cell Architecture.Frontiers in cell and developmental biology · 2019Review
- Tubulin Post-Translational Modifications and Microtubule Dynamics.International journal of molecular sciences · 2017Review
- The Central Apparatus of Cilia and Eukaryotic Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Article
- Discovery and functional evaluation of ciliary proteins in Tetrahymena thermophila.Methods in enzymology · 2013Article
- Ciliogenesis: building the cell's antenna.Nature reviews. Molecular cell biology · 2011Review
- Tubulin tyrosine ligase-like genes ttll3 and ttll6 maintain zebrafish cilia structure and motility.The Journal of biological chemistry · 2011Article
- Post-translational modifications of microtubules.Journal of cell science · 2010Review
- Hyperglutamylation of tubulin can either stabilize or destabilize microtubules in the same cell.Eukaryotic cell · 2010Article
- Basal body assembly in ciliates: the power of numbers.Traffic (Copenhagen, Denmark) · 2009Review
- Basal body components exhibit differential protein dynamics during nascent basal body assembly.Molecular biology of the cell · 2009Article
- Glutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena thermophila.Eukaryotic cell · 2008Article
- Polyglutamylation: a fine-regulator of protein function? 'Protein Modifications: beyond the usual suspects' review series.EMBO reports · 2008Review
- Tubulin modifications and their cellular functions.Current opinion in cell biology · 2008Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Tubulin glycylation is a posttranslational modification found in cells with cilia or flagella. The ciliate Tetrahymena has glycylation on ciliary and cortical microtubules. We showed previously that mutating three glycylation sites on beta-tubulin produces immotile 9 + 0 axonemes and inhibits cytokinesis. Here, we use an inducible glycylation domain mutation and epitope tagging to evaluate the potential of glycylation-deficient tubulin for assembly and maintenance of microtubular systems. In axonemes, the major defects, including lack of the central pair, occurred during assembly, and newly made cilia were abnormally short. The glycylation domain also was required for maintenance of the length of already assembled cilia. In contrast to the aberrant assembly of cilia, several types of cortical organelles showed an abnormally high number of microtubules in the same mutant cells. Thus, the consequences of deficiency in tubulin glycylation are organelle type specific and lead to either insufficient assembly (cilia) or excessive assembly (basal bodies and cortical microtubules). We suggest that the diverse functions of the beta-tubulin glycylation domain are executed by spatially restricted microtubule-associated proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.