ArticleMolecular biology of the cell2004
Cdc2 kinase-dependent disassembly of endoplasmic reticulum (ER) exit sites inhibits ER-to-Golgi vesicular transport during mitosis.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 58 citations in OpenAlex.
- Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis.Nature structural & molecular biology · 2024Article
- Fine-tuning cell organelle dynamics during mitosis by small GTPases.Frontiers of medicine · 2022Review
- Characteristics and Functions of the Yip1 Domain Family (YIPF), Multi-Span Transmembrane Proteins Mainly Localized to the Golgi Apparatus.Frontiers in cell and developmental biology · 2019Review
- Establishment and phenotyping of disease model cells created by cell-resealing technique.Scientific reports · 2017Article
- The G1 Cyclin-dependent Kinase CRK1 in Trypanosoma brucei Regulates Anterograde Protein Transport by Phosphorylating the COPII Subunit Sec31.The Journal of biological chemistry · 2016Article
- Mechanisms and Regulation of the Mitotic Inheritance of the Golgi Complex.Frontiers in cell and developmental biology · 2015Review
- VAMP4 is required to maintain the ribbon structure of the Golgi apparatus.Molecular and cellular biochemistry · 2013Article
- Multi-step down-regulation of the secretory pathway in mitosis: a fresh perspective on protein trafficking.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
- A resealed-cell system for analyzing pathogenic intracellular events: perturbation of endocytic pathways under diabetic conditions.PloS one · 2012Article
- Unraveling the Golgi ribbon.Traffic (Copenhagen, Denmark) · 2010Review
- VCP mutations causing frontotemporal lobar degeneration disrupt localization of TDP-43 and induce cell death.The Journal of biological chemistry · 2009Article
- CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation.BMC developmental biology · 2009Article
- Myt1 protein kinase is essential for Golgi and ER assembly during mitotic exit.The Journal of cell biology · 2008Article
- A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas.The Journal of cell biology · 2007Article
- Exit from mitosis triggers Chs2p transport from the endoplasmic reticulum to mother-daughter neck via the secretory pathway in budding yeast.The Journal of cell biology · 2006Article
- Nm23H2 facilitates coat protein complex II assembly and endoplasmic reticulum export in mammalian cells.Molecular biology of the cell · 2005Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We observed the disassembly of endoplasmic reticulum (ER) exit sites (ERES) by confocal microscopy during mitosis in Chinese hamster ovary (CHO) cells by using Yip1A fused to green fluorescence protein (GFP) as a transmembrane marker of ERES. Photobleaching experiments revealed that Yip1A-GFP, which was restricted to the ERES during interphase, diffused throughout the ER network during mitosis. Next, we reconstituted mitotic disassembly of Yip1A-GFP-labeled ERES in streptolysin O-permeabilized CHO cells by using mitotic L5178Y cytosol. Using the ERES disassembly assay and the anterograde transport assay of GFP-tagged VSVGts045, we demonstrated that the phosphorylation of p47 by Cdc2 kinase regulates the disassembly of ERES and results in the specific inhibition of ER-to-Golgi transport during mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.