ArticleJournal of virology2004
Cytokine-mediated downregulation of coxsackievirus-adenovirus receptor in endothelial cells.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 67 citations in OpenAlex.
- Contributions of coxsackievirus adenovirus receptor to tumorigenesis.Biochemical Society transactions · 2023Article
- TNIK regulation of interferon signaling and endothelial cell response to virus infection.Frontiers in cardiovascular medicine · 2023Article
- JAML promotes CD8 and γδ T cell antitumor immunity and is a novel target for cancer immunotherapy.The Journal of experimental medicine · 2021Article
- Vascular Endothelial Integrity Affects the Severity of Enterovirus-Mediated Cardiomyopathy.International journal of molecular sciences · 2021Article
- Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress.Experimental & molecular medicine · 2019Article
- CEACAM-1 promotes myocardial injury following coxsackievirus infection by regulating the coxsackievirus-adenovirus receptor.Medicine · 2019Article
- Secondary Placental Defects inFrontiers in physiology · 2019Article
- What is CAR doing in the middle of the adult neurogenic road?Neurogenesis (Austin, Tex.) · 2017Article
- Coxsackievirus Adenovirus Receptor Loss Impairs Adult Neurogenesis, Synapse Content, and Hippocampus Plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Influence of cell physiological state on gene delivery to T lymphocytes by chimeric adenovirus Ad5F35.Scientific reports · 2016Article
- Coxsackie-adenovirus receptor expression is enhanced in pancreas from patients with type 1 diabetes.BMJ open diabetes research & care · 2016Article
- JAM-related proteins in mucosal homeostasis and inflammation.Seminars in immunopathology · 2014Review
- Targeting adenovirus gene delivery to activated tumour-associated vasculature via endothelial selectins.Journal of controlled release : official journal of the Controlled Release Society · 2011Article
- Multiple phenotypes in adult mice following inactivation of the Coxsackievirus and Adenovirus Receptor (Car) gene.PloS one · 2011Article
- Autoimmunity in Coxsackievirus B3 induced myocarditis: role of estrogen in suppressing autoimmunity.Future virology · 2010Article
- Cytokine determinants of viral tropism.Nature reviews. Immunology · 2009Review
- Guanylate-binding protein-1 is expressed at tight junctions of intestinal epithelial cells in response to interferon-gamma and regulates barrier function through effects on apoptosis.Mucosal immunology · 2009Article
- Ephrin-B2 expression critically influences Nipah virus infection independent of its cytoplasmic tail.Virology journal · 2008Article
- Therapeutic potential of replication-selective oncolytic adenoviruses on cells from familial and sporadic desmoid tumors.Clinical cancer research : an official journal of the American Association for Cancer Research · 2008Article
- Coxsackie and adenovirus receptor (CAR) is a product of Sertoli and germ cells in rat testes which is localized at the Sertoli-Sertoli and Sertoli-germ cell interface.Experimental cell research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Endothelial cells have the ability to change their complement of cell surface proteins in response to inflammatory cytokines. We hypothesized that the expression of the coxsackievirus-adenovirus receptor (CAR), a viral receptor and putative cell-cell adhesion molecule, may be altered during the response of endothelial cells to inflammation. To test this hypothesis, we evaluated CAR protein and mRNA levels in human umbilical vein endothelial cells after they were exposed to tumor necrosis factor alpha, gamma interferon, or a combination of the two cytokines. Flow cytometric and Western blot analyses indicated that cytokine treatment led to a synergistic decrease in CAR protein expression. A Western blot analysis showed that CAR levels decreased to 16% +/- 4% or 1% +/- 4% of the CAR protein levels in untreated cells with either 24 or 48 h of cytokine treatment, respectively. Quantitative reverse transcription-PCR demonstrated that the combination treatment caused CAR mRNA levels to decrease to 21% +/- 12% or 5% +/- 3% of the levels in untreated cells after a 24- or 48-h cytokine treatment, respectively. Reduced CAR expression led to a decrease in adenovirus (Ad) binding of 80% +/- 3% (compared with untreated endothelial cells), with a subsequent decrease in Ad-mediated gene transfer that was dependent on the dose and duration of cytokine treatment but not on the dose of Ad. A similar decrease in CAR protein level and susceptibility to Ad infection was observed in human microvascular endothelial cells, while CAR expression on normal human bronchial epithelial cells or A549 lung epithelial cells was less affected by cytokine treatments. Taken together, the data demonstrate that inflammatory cytokines decrease CAR mRNA and protein expression with a concomitant decrease in Ad binding, reflecting the impact of cell physiology on the function of CAR and the potential effect of inflammation on the ability of Ad to transfer genes to endothelial cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.