ArticleProceedings of the National Academy of Sciences of the United States of America2004
Bipolar orientation of chromosomes in Saccharomyces cerevisiae is monitored by Mad1 and Mad2, but not by Mad3.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Differential roles for the spindle assembly checkpoint in error surveillance and mitotic timing.The EMBO journal · 2026Article
- Checkpoint Proteins Bub1 and Bub3 Delay Anaphase Onset in Response to Low Tension Independent of Microtubule-Kinetochore Detachment.Cell reports · 2019Article
- Absence of the Spindle Assembly Checkpoint Restores Mitotic Fidelity upon Loss of Sister Chromatid Cohesion.Current biology : CB · 2018Article
- Cdk1 phosphorylation of Esp1/Separase functions with PP2A and Slk19 to regulate pericentric Cohesin and anaphase onset.PLoS genetics · 2018Article
- Genetic instability in budding and fission yeast-sources and mechanisms.FEMS microbiology reviews · 2015Review
- Pericentromeric sister chromatid cohesion promotes kinetochore biorientation.Molecular biology of the cell · 2009Article
- Analysis of quality control substrates in distinct cellular compartments reveals a unique role for Rpn4p in tolerating misfolded membrane proteins.Molecular biology of the cell · 2009Article
- The spindle assembly checkpoint in Caenorhabditis elegans: one who lacks Mad1 becomes mad one.Cell cycle (Georgetown, Tex.) · 2009Article
- Key players in chromosome segregation in Caenorhabditis elegans.Frontiers in bioscience (Landmark edition) · 2009Review
- Heterozygous screen in Saccharomyces cerevisiae identifies dosage-sensitive genes that affect chromosome stability.Genetics · 2008Article
- Ybp2 associates with the central kinetochore of Saccharomyces cerevisiae and mediates proper mitotic progression.PloS one · 2008Article
- Article
- Synthetic lethal interactions identify phenotypic "interologs" of the spindle assembly checkpoint components.Genetics · 2007Article
- Novel sfi1 alleles uncover additional functions for Sfi1p in bipolar spindle assembly and function.Molecular biology of the cell · 2007Article
- Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores.Genes & development · 2007Article
- TagSmart: analysis and visualization for yeast mutant fitness data measured by tag microarrays.BMC bioinformatics · 2007Article
- The N-terminus of rodent and human MAD1 confers species-specific stringency to spindle assembly checkpoint.Oncogene · 2006Article
- A genomewide screen for petite-negative yeast strains yields a new subunit of the i-AAA protease complex.Molecular biology of the cell · 2006Article
- Article
- Improved statistical analysis of budding yeast TAG microarrays revealed by defined spike-in pools.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spindle checkpoint governs the timing of anaphase separation of sister chromatids. In budding yeast, Mad1, Mad2, and Mad3 proteins are equally required for arrest in the presence of damage induced by antimicrotubule drugs or catastrophic loss of spindle structure. We find that the MAD genes are not equally required for robust growth in the presence of more subtle kinetochore and microtubule damage. A mad1Delta synthetic lethal screen identified 16 genes whose deletion in cells lacking MAD1 results in death or slow growth. Eleven of these mad1Delta genetic interaction partners encode proteins at the kinetochore-microtubule interface. Analysis of the entire panel revealed similar phenotypes in combination with mad2Delta. In contrast, 13 panel mutants exhibited a less severe phenotype in combination with mad3Delta. Checkpoint arrest in the absence of bipolar orientation and tension (induced by replication block in a cdc6 mutant) was lacking in cells without MAD1 or MAD2. Cells without MAD3 were checkpoint-proficient. We conclude that Mad1 and Mad2 are required to detect bipolar orientation and/or tension at kinetochores, whereas Mad3 is not.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.