ArticleNucleic acids research2004
Measurement of DNA mismatch repair activity in live cells.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Divergent Roles of Canonical and Non-Canonical Mismatch Repair in Regulating Temozolomide Sensitivity in Glioblastoma.International journal of molecular sciences · 2026Review
- Comprehensive Measurement of Inter-Individual Variation in DNA Repair Capacity in Healthy Individuals.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters.Nature protocols · 2021Article
- Fluorescence Sheds Light on DNA Damage, DNA Repair, and Mutations.Trends in cancer · 2021Review
- EGFRvIII upregulates DNA mismatch repair resulting in increased temozolomide sensitivity of MGMT promoter methylated glioblastoma.Oncogene · 2020Article
- DNA mismatch repair is required for the host innate response and controls cellular fate after influenza virus infection.Nature microbiology · 2019Article
- Fluorescent Probes of DNA Repair.ACS chemical biology · 2018Review
- Acetylation regulates DNA repair mechanisms in human cells.Cell cycle (Georgetown, Tex.) · 2016Article
- Inter-individual variation in DNA repair capacity: a need for multi-pathway functional assays to promote translational DNA repair research.DNA repair · 2014Article
- The mechanism of mismatch repair and the functional analysis of mismatch repair defects in Lynch syndrome.Familial cancer · 2013Review
- Molecular signaling mechanisms of apoptosis in hereditary non-polyposis colorectal cancer.World journal of gastrointestinal pathophysiology · 2012Article
- DNA mismatch repair proficiency executing 5-fluorouracil cytotoxicity in colorectal cancer cells.Cancer biology & therapy · 2011Article
- Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Gene silencing induced by oxidative DNA base damage: association with local decrease of histone H4 acetylation in the promoter region.Nucleic acids research · 2010Article
- Rapid isolation of highly pure single-stranded DNA from phagemids.Analytical biochemistry · 2009Article
- Preparation of heteroduplex enhanced green fluorescent protein plasmid for in vivo mismatch repair activity assay.Analytical biochemistry · 2009Article
- Article
- Rad9 plays an important role in DNA mismatch repair through physical interaction with MLH1.Nucleic acids research · 2008Article
- HOXA5 regulates hMLH1 expression in breast cancer cells.Neoplasia (New York, N.Y.) · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Loss of DNA mismatch repair (MMR) function leads to the development and progression of certain cancers. Currently, assays for DNA MMR activity involve the use of cell extracts and are technically challenging and costly. Here, we report a rapid, less labor-intensive method that can quantitatively measure MMR activity in live cells. A G-G or T-G mismatch was introduced into the ATG start codon of the enhanced green fluorescent protein (EGFP) gene. Repair of the G-G or T-G mismatch to G-C or T-A, respectively, in the heteroduplex plasmid generates a functional EGFP gene expression. The heteroduplex plasmid and a similarly constructed homoduplex plasmid were transfected in parallel into the same cell line and the number of green cells counted by flow cytometry. Relative EGFP expression was calculated as the total fluorescence intensity of cells transfected with the heteroduplex construct divided by that of cells transfected with the homoduplex construct. We have tested several cell lines from both MMR-deficient and MMR-proficient groups using this method, including a colon carcinoma cell line HCT116 with defective hMLH1 gene and a derivative complemented by transient transfection with hMLH1 cDNA. Results show that MMR-proficient cells have significantly higher EGFP expression than MMR-deficient cells, and that transient expression of hMLH1 alone can elevate MMR activity in HCT116 cells. This method is potentially useful in comparing and monitoring MMR activity in live cells under various growth conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.