ArticlePlant physiology2004
Identification and characterization of stretch-activated ion channels in pollen protoplasts.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 174 citations in OpenAlex.
- Mechanosensitive ion channel MSL8 is required for oscillatory growth and cell wall dynamics in Arabidopsis pollen tubes.Plant reproduction · 2025Article
- Expression of Clementine Asp-Rich Proteins (CcASP-RICH) in Tobacco Plants Interferes with the Mechanism of Pollen Tube Growth.International journal of molecular sciences · 2022Article
- Calmodulin Domain Protein Kinase PiCDPK1 Regulates Pollen Tube Growth Polarity through Interaction with RhoGDI.Plants (Basel, Switzerland) · 2022Article
- Evolutionary and Regulatory Pattern Analysis of Soybean CaFrontiers in plant science · 2022Article
- P2K1 Receptor, Heterotrimeric Gα Protein and CNGC2/4 Are Involved in Extracellular ATP-Promoted Ion Influx in the Pollen ofPlants (Basel, Switzerland) · 2021Article
- The Role of Calcium/Calcium-Dependent Protein Kinases Signal Pathway in Pollen Tube Growth.Frontiers in plant science · 2021Review
- Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth.Plants (Basel, Switzerland) · 2020Review
- Signaling in Pollen Tube Growth: Beyond the Tip of the Polarity Iceberg.Plants (Basel, Switzerland) · 2019Review
- Extracellular ATP promoted pollen germination and tube growth of Nicotiana tabacum through promoting KPlant reproduction · 2018Article
- Lily Pollen Tubes Pulse According to a Simple Spatial Oscillator.Scientific reports · 2018Article
- Calcium dynamics in tomato pollen tubes using the Yellow Cameleon 3.6 sensor.Plant reproduction · 2018Article
- Simultaneous imaging and functional studies reveal a tight correlation between calcium and actin networks.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth.Plant physiology · 2018Review
- A mechanosensitive CaNature communications · 2017Article
- Perturbation Analysis of Calcium, Alkalinity and Secretion during Growth of Lily Pollen Tubes.Plants (Basel, Switzerland) · 2016Article
- Cyclic nucleotide-gated channel 18 is an essential Ca2+ channel in pollen tube tips for pollen tube guidance to ovules in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Pollen Aquaporins: The Solute Factor.Frontiers in plant science · 2016Review
- Mechanosensitive channel MSL8 regulates osmotic forces during pollen hydration and germination.Science (New York, N.Y.) · 2015Article
- Are Aquaporins the Missing Transmembrane Osmosensors?The Journal of membrane biology · 2015Review
- A calcium sensor-regulated protein kinase, CALCINEURIN B-LIKE PROTEIN-INTERACTING PROTEIN KINASE19, is required for pollen tube growth and polarity.Plant physiology · 2015Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen tube growth requires a Ca2+ gradient, with elevated levels of cytosolic Ca2+ at the growing tip. This gradient's magnitude oscillates with growth oscillation but is always maintained. Ca2+ influx into the growing tip is necessary, and its magnitude also oscillates with growth. It has been widely assumed that stretch-activated Ca2+ channels underlie this influx, but such channels have never been reported in either pollen grains or pollen tubes. We have identified and characterized stretch-activated Ca2+ channels from Lilium longiflorum pollen grain and tube tip protoplasts. The channels were localized to a small region of the grain protoplasts associated with the site of tube germination. In addition, we find a stretch-activated K+ channel as well as a spontaneous K+ channel distributed over the entire grain surface, but neither was present at the germination site or at the tip. Neither stretch-activated channel was detected in the grain protoplasts unless the grains were left in germination medium for at least 1 h before protoplast preparation. The stretch-activated channels were inhibited by a spider venom that is known to block stretch-activated channels in animal cells, but the spontaneous channel was unaffected by the venom. The venom also stopped pollen tube germination and elongation and blocked Ca2+ entry into the growing tip, suggesting that channel function is necessary for growth.
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