ArticlePlant physiology2004
Autophosphorylation and subcellular localization dynamics of a salt- and water deficit-induced calcium-dependent protein kinase from ice plant.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 105 citations in OpenAlex.
- OmicIntegrator: A Simple and Versatile Tool for Meta-Analysis.Plants (Basel, Switzerland) · 2026Article
- Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis.Communications biology · 2022Article
- Phosphorylation-dependent subfunctionalization of the calcium-dependent protein kinase CPK28.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- CaInternational journal of molecular sciences · 2021Article
- Actin Cytoskeleton as Actor in Upstream and Downstream of Calcium Signaling in Plant Cells.International journal of molecular sciences · 2019Review
- S-acylation mediates Mungbean yellow mosaic virus AC4 localization to the plasma membrane and in turns gene silencing suppression.PLoS pathogens · 2018Article
- TheInternational journal of molecular sciences · 2018Review
- Overexpression of a constitutively active truncated form of OsCDPK1 confers disease resistance by affecting OsPR10a expression in rice.Scientific reports · 2018Article
- Genome-Wide Analysis of CDPK Family in Foxtail Millet and Determination ofFrontiers in plant science · 2018Article
- Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans.Plant cell reports · 2017Article
- Autophosphorylation-based Calcium (CaThe Journal of biological chemistry · 2017Article
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- Genome-Wide Identification and Expression Analysis of Calcium-dependent Protein Kinase in Tomato.Frontiers in plant science · 2016Article
- A Role for Barley Calcium-Dependent Protein Kinase CPK2a in the Response to Drought.Frontiers in plant science · 2016Article
- Functions of Calcium-Dependent Protein Kinases in Plant Innate Immunity.Plants (Basel, Switzerland) · 2014Review
- The myristoylated amino-terminus of an Arabidopsis calcium-dependent protein kinase mediates plasma membrane localization.Plant molecular biology · 2013Article
- Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings.Plant molecular biology · 2013Article
- CDPKs in immune and stress signaling.Trends in plant science · 2013Review
- Abscisic acid and CO2 signalling via calcium sensitivity priming in guard cells, new CDPK mutant phenotypes and a method for improved resolution of stomatal stimulus-response analyses.Annals of botany · 2012Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
A salinity and dehydration stress-responsive calcium-dependent protein kinase (CDPK) was isolated from the common ice plant (Mesembryanthemum crystallinum; McCPK1). McCPK1 undergoes myristoylation, but not palmitoylation in vitro. Removal of the N-terminal myristate acceptor site partially reduced McCPK1 plasma membrane (PM) localization as determined by transient expression of green fluorescent protein fusions in microprojectile-bombarded cells. Removal of the N-terminal domain (amino acids 1-70) completely abolished PM localization, suggesting that myristoylation and possibly the N-terminal domain contribute to membrane association of the kinase. The recombinant, Escherichia coli-expressed, full-length McCPK1 protein was catalytically active in a calcium-dependent manner (K0.5 = 0.15 microm). Autophosphorylation of recombinant McCPK1 was observed in vitro on at least two different Ser residues, with the location of two sites being mapped to Ser-62 and Ser-420. An Ala substitution at the Ser-62 or Ser-420 autophosphorylation site resulted in a slight increase in kinase activity relative to wild-type McCPK1 against a histone H1 substrate. In contrast, Ala substitutions at both sites resulted in a dramatic decrease in kinase activity relative to wild-type McCPK1 using histone H1 as substrate. McCPK1 undergoes a reversible change in subcellular localization from the PM to the nucleus, endoplasmic reticulum, and actin microfilaments of the cytoskeleton in response to reductions in humidity, as determined by transient expression of McCPK1-green fluorescent protein fusions in microprojectile-bombarded cells and confirmed by subcellular fractionation and western-blot analysis of 6x His-tagged McCPK1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.