ArticlePlant physiology2004
Targeted modification of homogalacturonan by transgenic expression of a fungal polygalacturonase alters plant growth.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 68 citations in OpenAlex.
- Ectopic expression of pectate lyaseiScience · 2025Article
- Building an extensible cell wall.Plant physiology · 2022Article
- Exploring the potential of engineering polygalacturonase-inhibiting protein as an ecological, friendly, and nontoxic pest control agent.Biotechnology and bioengineering · 2021Article
- Host Cell Wall Damage during Pathogen Infection: Mechanisms of Perception and Role in Plant-Pathogen Interactions.Plants (Basel, Switzerland) · 2021Review
- Impaired Cuticle Functionality and Robust Resistance toFrontiers in plant science · 2021Article
- Expression of a Hyperthermophilic Cellobiohydrolase in TransgenicPlants (Basel, Switzerland) · 2020Article
- Post-Synthetic Reduction of Pectin Methylesterification Causes Morphological Abnormalities and Alterations to Stress Response inPlants (Basel, Switzerland) · 2020Article
- Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass.Frontiers in plant science · 2020Review
- Decreased Polysaccharide Feruloylation Compromises Plant Cell Wall Integrity and Increases Susceptibility to Necrotrophic Fungal Pathogens.Frontiers in plant science · 2016Article
- The Arabidopsis Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.Plant physiology · 2015Article
- Production and characterization of in planta transiently produced polygalacturanase from Aspergillus niger and its fusions with hydrophobin or ELP tags.BMC biotechnology · 2014Article
- Analysis of pectin mutants and natural accessions of Arabidopsis highlights the impact of de-methyl-esterified homogalacturonan on tissue saccharification.Biotechnology for biofuels · 2013Article
- Cell wall integrity: targeted post-synthetic modifications to reveal its role in plant growth and defense against pathogens.Plant signaling & behavior · 2013Review
- Down-regulation of POLYGALACTURONASE1 alters firmness, tensile strength and water loss in apple (Malus x domestica) fruit.BMC plant biology · 2012Article
- A gene for plant protection: expression of a bean polygalacturonase inhibitor in tobacco confers a strong resistance against Rhizoctonia solani and two oomycetes.Frontiers in plant science · 2012Article
- Post-synthetic modification of plant cell walls by expression of microbial hydrolases in the apoplast.Plant molecular biology · 2011Article
- Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Transgenic expression of a fungal endo-polygalacturonase increases plant resistance to pathogens and reduces auxin sensitivity.Plant physiology · 2008Article
- Reduced content of homogalacturonan does not alter the ion-mediated increase in xylem hydraulic conductivity in tobacco.Plant physiology · 2007Article
- Polygalacturonase-inhibiting protein interacts with pectin through a binding site formed by four clustered residues of arginine and lysine.Plant physiology · 2006Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pectins are a highly complex family of cell wall polysaccharides comprised of homogalacturonan (HGA), rhamnogalacturonan I and rhamnogalacturonan II. We have specifically modified HGA in both tobacco (Nicotiana tabacum) and Arabidopsis by expressing the endopolygalacturonase II of Aspergillus niger (AnPGII). Cell walls of transgenic tobacco plants showed a 25% reduction in GalUA content as compared with the wild type and a reduced content of deesterified HGA as detected by antibody labeling. Neutral sugars remained unchanged apart from a slight increase of Rha, Ara, and Gal. Both transgenic tobacco and Arabidopsis were dwarfed, indicating that unesterified HGA is a critical factor for plant cell growth. The dwarf phenotypes were associated with AnPGII activity as demonstrated by the observation that the mutant phenotype of tobacco was completely reverted by crossing the dwarfed plants with plants expressing PGIP2, a strong inhibitor of AnPGII. The mutant phenotype in Arabidopsis did not appear when transformation was performed with a gene encoding AnPGII inactivated by site directed mutagenesis.
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