ArticleNucleic acids research2004
A phage display selection of engrailed homeodomain mutants and the importance of residue Q50.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Diversifying the structure of zinc finger nucleases for high-precision genome editing.Nature communications · 2019Article
- Selection of heptapeptides that bind helix 69 of bacterial 23S ribosomal RNA.Bioorganic & medicinal chemistry · 2013Article
- Probing the informational and regulatory plasticity of a transcription factor DNA-binding domain.PLoS genetics · 2012Article
- The Homeodomain Resource: a comprehensive collection of sequence, structure, interaction, genomic and functional information on the homeodomain protein family.Database : the journal of biological databases and curation · 2009Article
- A one-dimensional reaction coordinate for identification of transition states from explicit solvent P(fold)-like calculations.Biophysical journal · 2007Article
- Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method.Nucleic acids research · 2007Article
- Sequence specificity of single-stranded DNA-binding proteins: a novel DNA microarray approach.Nucleic acids research · 2007Article
- Homeodomain revisited: a lesson from disease-causing mutations.Human genetics · 2005Review
- Reprogramming cells with synthetic proteins.Asian journal of andrologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Mutants of engrailed homeodomain (HD) that retain DNA-binding activity were isolated using a phage display selection. This selection was used to enrich for active DNA-binding clones from a complex library consisting of over a billion members. A more focused library of mutant homeodomains consisting of all possible amino acid combinations at two DNA-contacting residues (I47 and Q50) was constructed and screened for members capable of binding tightly and specifically to the engrailed consensus sequence, TAATTA. The isolated mutants largely recapitulated the distribution of amino acids found at these positions in natural homeodomains thus validating the in vitro selection conditions. In particular, the unequivocal advantage enjoyed by glutamine at residue 50 is surprising in light of reports that minimize the importance of this residue. Here, the subtle contributions of residue Q50 are demonstrated to play a functionally important role in specific recognition of DNA. These results highlight the complex subtlety of protein-DNA interactions, underscoring the value of the first reported in vitro selection of a homeodomain.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.