ArticleNucleic acids research2004
A novel 4-base-recognizing RNA cutter that can remove the single 3' terminal nucleotides from RNA molecules.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Human small non-coding tRNA-derived fragments as key hallmarks of host-virus interaction in viral infections.Cell & bioscience · 2026Review
- tRNA-derived fragments in gastric cancer: Biomarkers and functions.Journal of cellular and molecular medicine · 2022Review
- TRUE Gene Silencing.International journal of molecular sciences · 2022Review
- Deciphering the tRNA-derived small RNAs: origin, development, and future.Cell death & disease · 2021Review
- The heptamer sgRNA targeting the human OCT4 mRNA can upregulate the OCT4 expression.Biochemistry and biophysics reports · 2021Article
- The non-coding epitranscriptome in cancer.Briefings in functional genomics · 2021Review
- The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer.Frontiers in oncology · 2020Article
- The dysregulation of tRNAs and tRNA derivatives in cancer.Journal of experimental & clinical cancer research : CR · 2018Review
- SIDT2 mediates gymnosis, the uptake of naked single-stranded oligonucleotides into living cells.RNA biology · 2017Article
- TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents.Journal of visualized experiments : JoVE · 2016Article
- Potential small guide RNAs for tRNase ZL from human plasma, peripheral blood mononuclear cells, and cultured cell lines.PloS one · 2015Article
- Growth inhibition of head and neck squamous cell carcinoma cells by sgRNA targeting the cyclin D1 mRNA based on TRUE gene silencing.PloS one · 2014Article
- Elimination of specific miRNAs by naked 14-nt sgRNAs.PloS one · 2012Article
- Article
- Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA.Gene therapy · 2007Article
- Optimization and characterization of tRNA-shRNA expression constructs.Nucleic acids research · 2007Article
- Inhibition of HIV-1 gene expression by retroviral vector-mediated small-guide RNAs that direct specific RNA cleavage by tRNase ZL.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian tRNase ZL shows versatility in substrate recognition. This enzyme can not only process pre-tRNAs by cleaving off their 3' trailer sequences, but also recognize and cleave pre-tRNA-like complexes and micro-pre-tRNAs. Here we demonstrate that 24-27 nt hairpin RNAs (hook RNAs) can guide cleavages of separate target RNAs by tRNase ZL through the micro-pre-tRNA-like complexes between the targets and the hook RNAs and that tRNase ZL together with hook RNA works as 4-7-base-recognizing RNA cutters. The cleavage sites were located only after the nucleotide corresponding to the discriminator nucleotide. Cleavage assays for various substrate/hooker complexes showed that the cleavage efficiency changes depending on the maximum number of substrate/hooker recognition base pairings and the stem length of hook RNA and that a 5 nt recognition sequence and a hook RNA containing a 6 or 7 bp stem are the best combination for the optimal target cleavage. We also show that a 4-base RNA cutter can remove the single 3' terminal nucleotides from RNA molecules. These results indicate that this new type of RNA cutter can be utilized to homogenize at their 3' termini RNA transcripts synthesized in vitro with a bacteriophage RNA polymerase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.