ArticleGut2004
Altered glucose metabolism and proteolysis in pancreatic cancer cell conditioned myoblasts: searching for a gene expression pattern with a microarray analysis of 5000 skeletal muscle genes.
Article in Gut, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Glucose metabolic phenotype of pancreatic cancer.World journal of gastroenterology · 2016Pooled it
- Article
- Mitochondrial Genetic and Epigenetic Regulations in Cancer: Therapeutic Potential.International journal of molecular sciences · 2022Review
- Updated risk factors to inform early pancreatic cancer screening and identify high risk patients.Cancer letters · 2020Article
- Relationship between pancreatic cancer-associated diabetes and cachexia.Journal of cachexia, sarcopenia and muscle · 2020Article
- In Silico Analysis of Gene Expression Change Associated with Copy Number of Enhancers in Pancreatic Adenocarcinoma.International journal of molecular sciences · 2019Article
- Article
- Type 3c (pancreatogenic) diabetes mellitus secondary to chronic pancreatitis and pancreatic cancer.The lancet. Gastroenterology & hepatology · 2016Review
- Pancreatic cancer cell-derived IGFBP-3 contributes to muscle wasting.Journal of experimental & clinical cancer research : CR · 2016Article
- Neutrophil-Derived Proteases in the Microenvironment of Pancreatic Cancer -Active Players in Tumor Progression.International journal of biological sciences · 2016Review
- Analysis of global gene expression profiles suggests a role of acute inflammation in type 3C diabetes mellitus caused by pancreatic ductal adenocarcinoma.Diabetologia · 2015Article
- The early diagnosis of pancreatic cancer and diabetes: what's the relationship?Journal of gastrointestinal oncology · 2014Review
- Baseline hemoglobin-A1c impacts clinical outcomes in patients with pancreatic cancer.Journal of the National Comprehensive Cancer Network : JNCCN · 2014Article
- New insights into pancreatic cancer-induced paraneoplastic diabetes.Nature reviews. Gastroenterology & hepatology · 2013Review
- Adrenomedullin is up-regulated in patients with pancreatic cancer and causes insulin resistance in β cells and mice.Gastroenterology · 2012Article
- The bidirectional interation between pancreatic cancer and diabetes.World journal of surgical oncology · 2012Review
- Article
- New-onset diabetes: a potential clue to the early diagnosis of pancreatic cancer.The Lancet. Oncology · 2009 · on this mapReview
- Prevalence and clinical profile of pancreatic cancer-associated diabetes mellitus.Gastroenterology · 2008Article
- Pancreatic cancer-associated diabetes mellitus: prevalence and temporal association with diagnosis of cancer.Gastroenterology · 2008Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
BACKGROUND AND
aimsWe verified whether conditioned media (CM) from pancreatic cancer cell lines (MIAPaCa2, CAPAN-1, PANC-1, BxPC3) alter glucose metabolism and gene expression profiles (microarray experiment with a platform of 5000 skeletal muscle cDNA) in mice myoblasts.
methodsMyoblasts were incubated with control or pancreatic cancer CM for 24 and 48 hours.
resultsLactate significantly increased in CM compared with non-conditioned myoblasts. No variations in expression levels of the main genes involved in glycolysis were found in CM myoblasts. Propionyl coenzyme A carboxylase and isocitrate dehydrogenase 3 beta genes, which encode enzymes of the tricarboxylic acid cycle, were overexpressed, while IGFIIR and VAMP5 genes were underexpressed in CM myoblasts. PAFAH1B1 and BCL-2 genes (intracellular signal transduction) and the serine protease cathepsin G (proteolysis), were overexpressed in CM myoblasts. Tyrosine accumulation in CM myoblasts suggested that proteolysis overcomes protein synthesis. Sorcin, actin alpha, troponin T1, and filamin A were underexpressed in CM myoblasts.
conclusionsOur findings demonstrate that pancreatic cancer cell conditioned media enhanced lactate production and induced proteolysis, possibly by altering expression levels of a large number of genes, not only those involved in protein biosynthesis and degradation or glucose metabolism, but also those involved in the tricarboxylic acid cycle and in vesicle traffic.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.