ArticleJournal of clinical microbiology2004
Detection and genotyping of Entamoeba histolytica, Entamoeba dispar, Giardia lamblia, and Cryptosporidium parvum by oligonucleotide microarray.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- High resolution melting real-time PCR for genotyping of Giardia lamblia assemblages A and B regardless of parasite load.Gut pathogens · 2025Article
- Establishment and preliminary application of PCR-RFLP genotyping method for Giardia duodenalis in goats.BMC veterinary research · 2024Article
- Development and Assessment of a Diagnostic DNA Oligonucleotide Microarray for Detection and Typing of Meningitis-Associated Bacterial Species.High-throughput · 2018Article
- PCR-RFLP method to detect zoonotic and host-specific Giardia duodenalis assemblages in dog fecal samples.Parasitology research · 2016Article
- Development of a rapid HRM genotyping method for detection of dog-derived Giardia lamblia.Parasitology research · 2015Article
- Laboratory methods of identification of Entamoeba histolytica and its differentiation from look-alike Entamoeba spp.Tropical parasitology · 2014Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Progress in the research on diagnosis and vaccines in amebiasis.Tropical parasitology · 2011Article
- Development of a direct DNA extraction protocol for real-time PCR detection of Giardia lamblia from surface water.Ecotoxicology (London, England) · 2009Article
- Progress of science from microscopy to microarrays (part 1): diagnosis of parasitic diseases.Journal of laboratory physicians · 2009Article
- Laboratory diagnostic techniques for Entamoeba species.Clinical microbiology reviews · 2007Review
- Molecular methods for diagnosis of Entamoeba histolytica in a clinical setting: an overview.Experimental parasitology · 2007Review
- Identifying influenza viruses with resequencing microarrays.Emerging infectious diseases · 2006Article
- Simultaneous detection of four human pathogenic microsporidian species from clinical samples by oligonucleotide microarray.Journal of clinical microbiology · 2005Article
- Multiplex real-time PCR assay using Scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples.Journal of clinical microbiology · 2005Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum are the most frequently identified protozoan parasites causing waterborne disease outbreaks. The morbidity and mortality associated with these intestinal parasitic infections warrant the development of rapid and accurate detection and genotyping methods to aid public health efforts aimed at preventing and controlling outbreaks. In this study, we describe the development of an oligonucleotide microarray capable of detecting and discriminating between E. histolytica, Entamoeba dispar, G. lamblia assemblages A and B, and C. parvum types 1 and 2 in a single assay. Unique hybridization patterns for each selected protozoan were generated by amplifying six to eight diagnostic sequences/organism by multiplex PCR; fluorescent labeling of the amplicons via primer extension; and subsequent hybridization to a set of genus-, species-, and subtype-specific covalently immobilized oligonucleotide probes. The profile-based specificity of this methodology not only permitted for the unequivocal identification of the six targeted species and subtypes, but also demonstrated its potential in identifying related species such as Cryptosporidium meleagridis and Cryptosporidium muris. In addition, sensitivity assays demonstrated lower detection limits of five trophozoites of G. lamblia. Taken together, the specificity and sensitivity of the microarray-based approach suggest that this methodology may provide a promising tool to detect and genotype protozoa from clinical and environmental samples.
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