ArticleJournal of clinical microbiology2004
Use of oligonucleotide microarrays for rapid detection and serotyping of acute respiratory disease-associated adenoviruses.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 67 citations in OpenAlex.
- Oligonucleotide therapy: An emerging focus area for drug delivery in chronic inflammatory respiratory diseases.Chemico-biological interactions · 2019Review
- Laboratory Diagnosis of Respiratory Tract Infections in Children - the State of the Art.Frontiers in microbiology · 2018Review
- Quantitative real-time PCR assays for detection and type-specific identification of the endemic species C human adenoviruses.Journal of virological methods · 2016Article
- Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review.World journal of gastroenterology · 2016Review
- Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples.BioMed research international · 2016Article
- DNA microarray for detection of gastrointestinal viruses.Journal of clinical microbiology · 2015Article
- Molecular identification of adenoviruses associated with respiratory infection in Egypt from 2003 to 2010.BMC infectious diseases · 2014Article
- Quantitative real-time PCR assay panel for detection and type-specific identification of epidemic respiratory human adenoviruses.Journal of clinical microbiology · 2013Article
- Simultaneous detection of major enteric viruses using a combimatrix microarray.Journal of microbiology (Seoul, Korea) · 2012Article
- Comparison of the GenMark Diagnostics eSensor respiratory viral panel to real-time PCR for detection of respiratory viruses in children.Journal of clinical microbiology · 2012Article
- Development of real-time PCR array for simultaneous detection of eight human blood-borne viral pathogens.PloS one · 2012Article
- Molecular diagnosis of viral respiratory infections.Current infectious disease reports · 2011Article
- A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools.Veterinary medicine international · 2011Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.Diagnostic microbiology and infectious disease · 2008Article
- Plastic polymers for efficient DNA microarray hybridization: application to microbiological diagnostics.Journal of clinical microbiology · 2008Article
- Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR.BMC molecular biology · 2008Article
- Detection of a broad range of human adenoviruses in respiratory tract samples using a sensitive multiplex real-time PCR assay.Journal of medical virology · 2008Article
- In situ-synthesized virulence and marker gene biochip for detection of bacterial pathogens in water.Applied and environmental microbiology · 2008Article
- Rapid detection and molecular serotyping of adenovirus by use of PCR followed by electrospray ionization mass spectrometry.Journal of clinical microbiology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cessation of the adenovirus vaccination program for military trainees has resulted in several recent acute respiratory disease (ARD) outbreaks. In the absence of vaccination, rapid detection methods are necessary for the timely implementation of measures to prevent adenovirus transmission within military training facilities. To this end, we have combined a fluorogenic real-time multiplex PCR assay with four sets of degenerate PCR primers that target the E1A, fiber, and hexon genes with a long oligonucleotide microarray capable of identifying the most common adenovirus serotypes associated with adult respiratory tract infections (serotypes 3, 4, 7, 16, and 21) and a representative member of adenovirus subgroup C (serotype 6) that is a common cause of childhood ARD and that often persists into adulthood. Analyses with prototype strains demonstrated unique hybridization patterns for representative members of adenovirus subgroups B(1), B(2), C, and E, thus allowing serotype determination. Microarray-based sensitivity assessments revealed lower detection limits (between 1 and 100 genomic copies) for adenovirus serotype 4 (Ad4) and Ad7 cell culture lysates, clinical nasal washes, and throat swabs and purified DNA from clinical samples. When adenovirus was detected from coded clinical samples, the results obtained by this approach demonstrated an excellent concordance with those obtained by the more established method of adenovirus identification as well as by cell culture with fluorescent-antibody staining. Finally, the utility of this method was further supported by its ability to detect adenoviral coinfections, contamination, and, potentially, recombination events. Taken together, the results demonstrate the usefulness of the simple and rapid diagnostic method developed for the unequivocal identification of ARD-associated adenoviral serotypes from laboratory or clinical samples that can be completed in 1.5 to 4.0 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.