ArticleJournal of clinical microbiology2004
Amplicon sequencing and improved detection of human rhinovirus in respiratory samples.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 78 citations in OpenAlex.
- Trial
- Sensitive detection and differentiation of rhinoviruses and enteroviruses by a nested real-time RT-PCR assay.Microbiology spectrum · 2026Article
- Reduction of Viral Load in Patients with Acute Sore Throats: Results from an Observational Clinical Trial with Echinacea/Salvia Lozenges.Complementary medicine research · 2023Observational
- Silver nanoparticles with plasma-polymerized hexamethyldisiloxane coating on 3D printed substrates are non-cytotoxic and effective against respiratory pathogens.Frontiers in microbiology · 2023Article
- Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma.Frontiers in bioengineering and biotechnology · 2022Article
- Detection of 12 respiratory viruses by duplex real time PCR assays in respiratory samples.Molecular and cellular probes · 2015Article
- Human respiratory syncytial virus in children with lower respiratory tract infections or influenza-like illness and its co-infection characteristics with viruses and atypical bacteria in Hangzhou, China.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2015Article
- Molecular epidemiology of rhinoviruses in Cyprus over three consecutive seasons.Epidemiology and infection · 2015Article
- Outbreak of coinfection with human metapneumovirus and measles virus resulting in the death of a child at a hospital in China.American journal of infection control · 2015Article
- Simultaneous detection and differentiation of human rhino- and enteroviruses in clinical specimens by real-time PCR with locked nucleic Acid probes.Journal of clinical microbiology · 2013Article
- Prospective surveillance study of acute respiratory infections, influenza-like illness and seasonal influenza vaccine in a cohort of juvenile idiopathic arthritis patients.Pediatric rheumatology online journal · 2013Article
- What is the clinical relevance of respiratory syncytial virus bronchiolitis?: findings from a multi-center, prospective study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Identification of site-specific adaptations conferring increased neural cell tropism during human enterovirus 71 infection.PLoS pathogens · 2012Article
- Rhinovirus frequently detected in elderly adults attending an emergency department.Journal of medical virology · 2011Article
- Rhinovirus C and respiratory exacerbations in children with cystic fibrosis.Emerging infectious diseases · 2010Article
- High rate of viral identification and coinfections in infants with acute bronchiolitis.Clinics (Sao Paulo, Brazil) · 2010Article
- A one-step, real-time PCR assay for rapid detection of rhinovirus.The Journal of molecular diagnostics : JMD · 2010Article
- Correlation of rhinovirus load in the respiratory tract and clinical symptoms in hospitalized immunocompetent and immunocompromised patients.Journal of medical virology · 2009Article
- Development of a RT real-time PCR for the detection and quantification of human rhinoviruses.Molecular biotechnology · 2009Article
- New molecular detection tools adapted to emerging rhinoviruses and enteroviruses.Journal of clinical microbiology · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improved knowledge of the genotypic characteristics of human rhinovirus (HRV) is required, as are nucleic detection assays with the capacity to overcome both the similarities between members of the family Picornaviridae and the wide diversity of different HRV serotypes. The goal of the present study was to investigate the variability and the genotypic diversity of clinical strains circulating in the community. Since most reverse transcription (RT)-PCR assays available cannot differentiate HRV from other members of the family Picornaviridae, we also validated an assay specific for HRV detection. The 5' noncoding regions of 87 different HRV serotypes and clinical isolates were sequenced. On the basis of sequence analysis and phylogenetic determination, we first confirmed that all clinical isolates were HRV. We then validated a real-time RT-PCR assay that was able not only to detect all HRV serotypes and all clinical isolates tested but also to accurately discriminate between rhinovirus and other viruses from the family Picornaviridae. This assay was negative with isolates of coxsackievirus (types A and B), echovirus, enterovirus, parechovirus, and poliovirus, as well as nonpicornaviruses. Among a series of bronchoalveolar lavage specimens, 4% (7 of 161) were positive by culture, whereas 13% (21 of 161) were positive by RT-PCR. In the present study we showed that to specifically identify HRV in clinical specimens, diagnostic assays need to overcome both the diversities and the similarities of picornaviruses. By sequencing the 5' noncoding regions of rhinoviruses recovered from clinical specimens, we designed probes that could specifically detect rhinovirus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.