ArticleJournal of clinical microbiology2004
Multiplex PCR assay for detection of Streptococcus suis species and serotypes 2 and 1/2 in tonsils of live and dead pigs.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 115 citations in OpenAlex.
- Development of a recombinase polymerase amplification nucleic acid lateral flow assay for detecting Streptococcus suis serotype 2 in pork.Scientific reports · 2025Article
- Novel multiplex PCR approach for the detection ofFrontiers in veterinary science · 2025Article
- Investigation of genomic and pathogenicity characteristics ofEmerging microbes & infections · 2024Article
- Contamination ofFoods (Basel, Switzerland) · 2024Article
- Isolation, Identification, Genomic Diversity, and Antimicrobial Resistance Analysis ofMicroorganisms · 2024Article
- Genomic epidemiology inHeliyon · 2024Review
- Ceftiofur treatment of sows results in long-term alterations in the nasal microbiota of the offspring that can be ameliorated by inoculation of nasal colonizers.Animal microbiome · 2023Article
- Article
- Virulence gene profile and antimicrobial resistance of non-typeable Streptococcus suis isolated from clinically healthy and diseased pigs from North East India.Folia microbiologica · 2022Article
- Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2Microbial genomics · 2022Article
- Epidemiology and Genetic Diversity of PCV2 Reveals That PCV2e Is an Emerging Genotype in Southern China: A Preliminary Study.Viruses · 2022Article
- Preparation of Aditoprim Injection againstPharmaceutics · 2022Article
- An Observational Study of Sepsis in Takeo Province Cambodia: An in-depth examination of pathogens causing severe infections.PLoS neglected tropical diseases · 2020Observational
- Pathotyping the Zoonotic Pathogen Streptococcus suis: Novel Genetic Markers To Differentiate Invasive Disease-Associated Isolates from Non-Disease-Associated Isolates from England and Wales.Journal of clinical microbiology · 2019Article
- Bovine vegetative endocarditis caused by Streptococcus suis.The Journal of veterinary medical science · 2018Article
- Novel Streptococcus suis Sequence Type 834 among Humans, Madagascar.Emerging infectious diseases · 2018Article
- Serotypes of Streptococcus suis isolated from healthy pigs in Phayao Province, Thailand.BMC research notes · 2017Article
- Evaluation of Galleria mellonella larvae for studying the virulence of Streptococcus suis.BMC microbiology · 2016Article
- Human meningitis due to Streptococcus suis in Lomé, Togo: a case report.BMC infectious diseases · 2016Article
- Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-Time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs.Pathogens (Basel, Switzerland) · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A PCR assay was developed for the detection of Streptococcus suis serotypes 2 and 1/2. This multiplex PCR is based on the amplification of the gene coding for 16S rRNA of S. suis and on the amplification of the cps2J gene coding for the capsule of S. suis serotypes 2 and 1/2. An internal control was constructed and added in this test to monitor the efficiency of amplification in each reaction. To evaluate the specificity of the test, 31 strains of other bacterial species related to S. suis or isolated from pigs and 42 strains of S. suis serotypes 1 and 3 to 34 were analyzed. The detection threshold of the test was 28 S. suis CFU/ml. The specificity and the sensitivity of the multiplex PCR test and the presence of an internal control allowed the analysis of biological samples without a culture step. The PCR assay was then applied to the detection of 14 S. suis serotype 1/2 strains, 88 S. suis serotype 2 strains isolated from pigs, and 25 S. suis serotype 2 strains isolated from humans. This test was also applied to analyze tonsil samples of pigs experimentally infected and carrier pigs without any symptoms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.