ArticleJournal of clinical microbiology2004
Multiplex real-time PCR for detection of anaplasma phagocytophilum and Borrelia burgdorferi.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 249 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
249 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Anaplasma Phagocytophilum, a Zoonotic Vector-Borne Bacterial Species in Rodents and Its Associated Tick Vector: Systematic Review.Veterinary medicine and science · 2025Pooled it
- The Roles of Contrasting Host Types on the Environmental Abundance ofEcology and evolution · 2026Article
- A recombinase polymerase amplification lateral flow assay (RPA-LF) for detection of Babesia divergens-like parasites.Parasites & vectors · 2026Article
- Asynchronous local differences in Lyme disease environmental hazard driven by variation in tick abundance and Borrelia burgdorferi infection prevalence.Parasites & vectors · 2026Article
- Theileria annulata diagnostics: past, present and future.Tropical animal health and production · 2026Review
- An in-house nucleic acid test for detection of Anaplasma phagocytophilum in whole blood donor samples.Transfusion · 2026Article
- Serological and Molecular Detection of Zoonotic Pathogens in European Bison (Pathogens (Basel, Switzerland) · 2026Article
- Pathogen screening of Zambian ticks: new insights on the occurrence of tick-borne pathogens in the country.Parasites & vectors · 2026Article
- Sympatric Occurrence of Five Exophilic Tick Species in the Levice Region (Southwestern Slovakia) and Their Infection with Tick-Borne Pathogens.Pathogens (Basel, Switzerland) · 2026Article
- Efficacy of RevolutionParasites & vectors · 2026Article
- Improving Molecular Detection of Tick-Borne Pathogens in Citizen-Collected Ticks.Pathogens (Basel, Switzerland) · 2026Article
- Single and Co-Infections by Tick-Borne Pathogens in Synanthropic European Hedgehogs (Veterinary sciences · 2026Article
- Molecular Detection ofPathogens (Basel, Switzerland) · 2026Article
- Stomoxys calcitrans as a potential mechanical vector of Anaplasma phagocytophilum: assessment through original ex vivo feeding models.Parasite (Paris, France) · 2026Article
- Exploration of geographical population structure ofCurrent research in parasitology & vector-borne diseases · 2026Article
- Assessment of human health risks to tick-borne infections in urban green spaces (UGS) - a study protocol.BMC infectious diseases · 2025Article
- Evaluation of Immune Response Dynamics: Analyzing the Parameters of Complete Blood Count (CBC) in Experimental Borreliosis.Life (Basel, Switzerland) · 2025Article
- Article
- Park Size and Trail Environment Influence the Seasonal Transmission Risk of Tick-Borne Pathogens in Nashville Metropolitan Area Parks, Tennessee.The American journal of tropical medicine and hygiene · 2025Article
- Review of Molecular Tools Used in Diagnosis ofAnimals : an open access journal from MDPI · 2025Review
189 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex real-time PCR assay was developed for the simultaneous detection of Anaplasma phagocytophilum and Borrelia burgdorferi. The assay was tested on various Anaplasma, Borrelia, Erhlichia, and Rickettsia species, as well as on Bartonella henselae and Escherichia coli, and the assay was found to be highly specific for A. phagocytophilum and the Borrelia species tested (B. burgdorferi, B. parkeri, B. andersonii, and B. bissettii). The analytical sensitivity of the assay is comparable to that of previously described nested PCR assays (A. phagocytophilum, 16S rRNA; B. burgdorferi, fla gene), amplifying the equivalent of one-eighth of an A. phagocytophilum-infected cell and 50 borrelia spirochetes. The dynamic range of the assay for both A. phagocytophilum and B. burgdorferi was >/=4 logs of magnitude. Purified DNA from A. phagocytophilum and B. burgdorferi was spiked into DNA extracted from uninfected ticks and from negative control mouse and human bloods, and these background DNAs were shown to have no significant effect on sensitivity or specificity of the assay. The assay was tested on field-collected Ixodes scapularis ticks and shown to have 100% concordance compared to previously described non-probe-based PCR assays. To our knowledge, this is the first report of a real-time multiplex PCR assay that can be used for the simultaneous and rapid screening of samples for A. phagocytophilum and Borrelia species, two of the most common tick-borne infectious agents in the United States.
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