ArticleJournal of clinical microbiology2004
Reference system for characterization of Bordetella pertussis pulsed-field gel electrophoresis profiles.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Characterization ofPathogens (Basel, Switzerland) · 2022Article
- Prevalence and characterization of pertactin deficientVaccine: X · 2021Article
- Genome diversity and evolutionary characteristics of clinical isolates ofIranian journal of microbiology · 2020Article
- Rare Detection of Bordetella pertussis Pertactin-Deficient Strains in Argentina.Emerging infectious diseases · 2019Article
- Genomic epidemiology of IranianEmerging microbes & infections · 2019Article
- Population dynamics and antigenic drift ofEmerging microbes & infections · 2019Article
- Strain variation and antigenic divergence among Bordetella pertussis circulating strains isolated from patients in Iran.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Surveillance of Circulating Bordetella pertussis Strains in Europe during 1998 to 2015.Journal of clinical microbiology · 2018Article
- The characterization of Bordetella pertussis strains isolated in the Central-Western region of Brazil suggests the selection of a specific genetic profile during 2012-2014 outbreaks.Epidemiology and infection · 2017Article
- Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012.BMC infectious diseases · 2016Article
- Changes in Predominance of Pulsed-Field Gel Electrophoresis Profiles of Bordetella pertussis Isolates, United States, 2000-2012.Emerging infectious diseases · 2016Article
- Isolation of High Level Macrolide Resistant Bordetella pertussis Without Transition Mutation at Domain V in Iran.Jundishapur journal of microbiology · 2015Article
- A Proteomic Characterization of Bordetella pertussis Clinical Isolates Associated with a California State Pertussis Outbreak.International journal of proteomics · 2015Article
- Genetic Profile Variation in Vaccine Strains and Clinical Isolates of Bordetella pertussis Recovered from Iranian Patients.Avicenna journal of medical biotechnology · 2014Article
- The First Macrolide-Resistant Bordetella pertussis Strains Isolated From Iranian Patients.Jundishapur journal of microbiology · 2014Article
- Appearance of macrolide-resistant Bordetella pertussis strains in China.Antimicrobial agents and chemotherapy · 2013Article
- Pulsed-field gel electrophoresis analysis of Bordetella pertussis isolates circulating in Europe from 1998 to 2009.Journal of clinical microbiology · 2013Article
- A gel-free proteomic-based method for the characterization of Bordetella pertussis clinical isolates.Journal of microbiological methods · 2012Article
- Pulsed-field gel electrophoresis characterization of Bordetella pertussis clinical isolates circulating in Turkey in 2001-2009.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Strain variation among Bordetella pertussis isolates circulating in Poland after 50 years of whole-cell pertussis vaccine use.Journal of clinical microbiology · 2011Article
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Authors and funding
3 authors.
Funding
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Abstract
Pulsed-field gel electrophoresis (PFGE) has been used as an epidemiological tool for surveillance studies of Bordetella pertussis since the early 1990s. To date there is no standardized procedure for comparison of results, and therefore it has been difficult to directly compare PFGE results between laboratories. We propose a profile-based reference system for PFGE characterization of B. pertussis strain variation and to establish traceability of B. pertussis PFGE results. We initially suggest 35 Swedish reference strains as reference material for PFGE traceability. This reference material is deposited at the Culture Collection of the University of Gothenburg, Gothenburg, Sweden. Altogether, 1,810 Swedish clinical isolates from between 1970 and 2003 were studied, together with the Swedish Pw vaccine strain, six reference strains, and two U.S. isolates. Our system provides evidence that profiles obtained by using only one enzyme, i.e., XbaI, give enough data to analyze the epidemiological relationship between them. Characterization with one enzyme is far less labor intensive, yielding results in half the time than when a two-enzyme procedure is used. Also, we can see that there is a correlation between PFGE profile and pertactin type. One common PFGE profile, BpSR11 (n = 455), showed 100% prn2 and 100% Fim3 when analyzed for pertactin type and serotype. On the other hand, strains with the same profile may express various serotypes when isolated over longer periods of time. Subculturing of the same isolate eight times or lyophilization caused no change in PFGE profile.
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