ArticleClinical and diagnostic laboratory immunology2004
Novel hairpin-shaped primer assay to study the association of the -44 single-nucleotide polymorphism of the DEFB1 gene with early-onset periodontal disease.
Article in Clinical and diagnostic laboratory immunology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 28 citations in OpenAlex.
- Gene polymorphism of β-defensin-1 is associated with susceptibility to periodontitis in Japanese.Odontology · 2015Article
- A Fast Method for DEFB1 - 44C/G SNP Genotyping in Brazilian Patients with Periodontitis.Acta stomatologica Croatica · 2014Article
- Variation in human β-defensin genes: new insights from a multi-population study.International journal of immunogenetics · 2013Article
- Clinical application of human β-defensin and CD14 gene polymorphism in evaluating the status of chronic inflammation.Journal of translational medicine · 2012Article
- Action of Coriandrum sativum L. Essential Oil upon Oral Candida albicans Biofilm Formation.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- A 3' UTR transition within DEFB1 is associated with chronic and aggressive periodontitis.Genes and immunity · 2010Article
- Defensins as anti-inflammatory compounds and mucosal adjuvants.Future microbiology · 2010Review
- Association of a genetic polymorphism (-44 C/G SNP) in the human DEFB1 gene with expression and inducibility of multiple beta-defensins in gingival keratinocytes.BMC oral health · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
A powerful, cost-effective new method for studying single-nucleotide polymorphisms (SNPs) is described. This method is based on the use of hairpin-shaped primers (HP), which give a sensitive and specific PCR amplification of each specific allele, without the use of costly fluorophore-labeled probes and any post-PCR manipulation. The amplification is monitored in real-time using SYBR Green I dye and takes only 2 h to yield results. The HP assay has a simple design and utilizes a conventional real-time PCR apparatus. The -44 C-->G transversion in the DEFB1 gene (which encodes human beta-defensin 1) has been previously associated with Candida carriage in oral epithelia. In this study, we analyzed the association between early-onset periodontal disease (EOP) and the -44 SNP. We used an HP assay to study the distribution of the -44 SNP in 264 human DNAs obtained from two cohorts of EOP patients and healthy controls from different ethnic backgrounds. The results indicate that the -44 SNP has a similar distribution between EOP and healthy patients, suggesting that it is not associated with the disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.