ArticleThe Biochemical journal2004
Upstream stimulatory factors, USF1 and USF2, bind to the human haem oxygenase-1 proximal promoter in vivo and regulate its transcription.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Diesel exhaust particle extract elicits an oxPAPC-like transcriptomic profile in macrophages across multiple mouse strains.Environmental pollution (Barking, Essex : 1987) · 2024Article
- To target or not to target? The role of DNA and histone methylation in bacterial infections.Epigenetics · 2023Review
- Irrelevance of USF2 rs916145 polymorphism with the risk of biliary atresia susceptibility in Southern Chinese children.Bioscience reports · 2020Article
- Interleukin-like EMT inducer (ILEI) promotes melanoma invasiveness and is transcriptionally up-regulated by upstream stimulatory factor-1 (USF-1).The Journal of biological chemistry · 2018Article
- Nrf2-AKT interactions regulate heme oxygenase 1 expression in kidney epithelia during hypoxia and hypoxia-reoxygenation.American journal of physiology. Renal physiology · 2016Article
- Integration of proteomic and transcriptomic profiles identifies a novel PDGF-MYC network in human smooth muscle cells.Cell communication and signaling : CCS · 2014Article
- Article
- Heme oxygenase-1 and acute kidney injury.Current opinion in nephrology and hypertension · 2014Review
- Upstream stimulatory factor 2 and hypoxia-inducible factor 2α (HIF2α) cooperatively activate HIF2 target genes during hypoxia.Molecular and cellular biology · 2012Article
- In vivo regulation of the heme oxygenase-1 gene in humanized transgenic mice.Kidney international · 2012Article
- Sp1 regulates chromatin looping between an intronic enhancer and distal promoter of the human heme oxygenase-1 gene in renal cells.The Journal of biological chemistry · 2010Article
- Human haem oxygenase-1 induction by nitro-linoleic acid is mediated by cAMP, AP-1 and E-box response element interactions.The Biochemical journal · 2009Article
- Target gene specificity of USF-1 is directed via p38-mediated phosphorylation-dependent acetylation.The Journal of biological chemistry · 2009Article
- Regulation of haeme oxygenase-1 for treatment of neuroinflammation and brain disorders.British journal of pharmacology · 2008Review
- Carbon monoxide and hydrogen sulfide: gaseous messengers in cerebrovascular circulation.Journal of applied physiology (Bethesda, Md. : 1985) · 2006Review
- The gene encoding the fragile X RNA-binding protein is controlled by nuclear respiratory factor 2 and the CREB family of transcription factors.Nucleic acids research · 2006Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The human HO-1 (haem oxygenase-1) gene encodes a microsomal enzyme responsible for the breakdown of haem, and is also cytoprotective in response to various cellular insults. HO-1 transcription is induced by a vast array of compounds including, but certainly not limited to, haem and heavy metals such as cadmium. In the present study, we show that upstream stimulatory factors, USF1 and USF2, ubiquitous proteins belonging to the basic helix-loop-helix-leucine zipper family of transcription factors, constitutively bind to the class B E-box located in the proximal promoter of the human HO-1 gene and are responsible for the enhancement of HO-1 gene transcription in human renal proximal tubular epithelial cells. Dimethylsulphate in vivo footprinting studies have identified three protected guanine residues in the E-box of the HO-1 proximal promoter. One of these guanine contact points is essential for USF binding, and when mutated mimics a deletion mutation of the entire E-box palindrome sequence encompassing all three guanine contact points. Binding of USF1 and USF2 to the HO-1 E-box was confirmed by chromatin immunoprecipitation and gel-shift assays. Furthermore, we show that overexpression of USF1 or USF2 enhances the basal expression of HO-1 and that expression of a USF dominant negative form reduces its expression. These results demonstrate for the first time that USF proteins bind to the human HO-1 promoter in vivo and are required for high-level expression of HO-1 by haem and cadmium in human renal epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.