ArticleThe Biochemical journal2004
A crustacean Ca2+-binding protein with a glutamate-rich sequence promotes CaCO3 crystallization.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Colloidal pathways of amorphous calcium carbonate formation lead to distinct water environments and conductivity.Nature communications · 2024Article
- Coral acid rich protein selects vaterite polymorph in vitro.Journal of structural biology · 2020Article
- Formation and Structure of Calcium Carbonate Thin Films and Nanofibers Precipitated in the Presence of Poly(Allylamine Hydrochloride) and Magnesium Ions.Chemistry of materials : a publication of the American Chemical Society · 2013Article
- Article
- Moult cycle specific differential gene expression profiling of the crab Portunus pelagicus.BMC genomics · 2011Article
- A gastrolith protein serving a dual role in the formation of an amorphous mineral containing extracellular matrix.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Gene expression profiling of cuticular proteins across the moult cycle of the crab Portunus pelagicus.BMC biology · 2007Article
- An IRE-like AGC kinase gene, MtIRE, has unique expression in the invasion zone of developing root nodules in Medicago truncatula.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DD4 mRNA of the penaeid prawn Penaeus japonicus was shown previously to be expressed in the epidermis adjacent to the exoskeleton specifically during the post-moult period, when calcification of the exoskeleton took place. The encoded protein possessed a Ca2+-binding site, suggesting its involvement in the calcification of the exoskeleton. In the present study, an additional ORF (open reading frame) of 289 amino acids was identified at the 5' end of the previous ORF. The newly identified part of the encoded protein included a region of approx. 120 amino acids that was highly rich in glutamate residues, and contained one or more Ca2+-binding sites. In an immunohistochemical study, signals were detected within calcified regions in the endocuticular layer of the exoskeleton. Bacterially expressed partial segments of the protein induced CaCO3 crystallization in vitro. Finally, a reverse transcription-PCR study showed that the expression was limited to an early part of the post-moult period, preceding significant calcification of the exoskeleton. These observations argue for the possibility that the encoded protein, renamed crustocalcin (CCN), promotes formation of CaCO3 crystals in the exoskeleton by inducing nucleation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.