ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2004
Generation of chromosome painting probes from single chromosomes by laser microdissection and linker-adaptor PCR.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- 2D and 3D chromosome painting in malaria mosquitoes.Journal of visualized experiments : JoVE · 2014Article
- Microdissection and chromosome painting of the alien chromosome in an addition line of wheat--Thinopyrum intermedium.PloS one · 2013Article
- Atomic Force Microscope nanolithography on chromosomes to generate single-cell genetic probes.Journal of nanobiotechnology · 2011Article
- Mapping of balanced chromosome translocation breakpoints to the basepair level from microdissected chromosomes.Journal of cellular and molecular medicine · 2010Article
- Nanomedicine--challenge and perspectives.Angewandte Chemie (International ed. in English) · 2009Review
- Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues.BMC biotechnology · 2008Article
- The development of chromosome microdissection and microcloning technique and its applications in genomic research.Current genomics · 2007Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence in situ hybridization (FISH) plays an essential role in research and clinical diagnostics. The versatility and resolution of FISH depends critically on the probe set used. Here, we describe an improved approach for the generation of specific DNA probes from single copies of chromosomes. Single chromosomes or single chromosomal regions were microdissected by laser pressure catapulting and amplified using linker-adaptor PCR. The probes were labeled and tested in various scenarios including multicolor-FISH experiments employing up to seven different fluorochromes. FISH confirmed the specific and even staining of the respective chromosomal regions. Furthermore, the capability of these probes to detect even small translocations (<3 Mb) suggests that the dissected regions are completely represented in the generated painting probes.
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