ArticleProceedings of the National Academy of Sciences of the United States of America2004
Lipid- and mechanosensitivities of sodium/hydrogen exchangers analyzed by electrical methods.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 83 citations in OpenAlex.
- Article
- Review
- Structure and elevator mechanism of the mammalian sodium/proton exchanger NHE9.The EMBO journal · 2020Article
- Acidic residues of extracellular loop 3 of the NaMolecular and cellular biochemistry · 2020Article
- Regulation of ion transport from within ion transit pathways.The Journal of general physiology · 2020Review
- Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters.Journal of visualized experiments : JoVE · 2018Article
- Fluorescent Visualization of Cellular Proton Fluxes.Cell chemical biology · 2016Article
- Sources of protons and a role for bicarbonate in inhibitory feedback from horizontal cells to cones in Ambystoma tigrinum retina.The Journal of physiology · 2016Article
- Tubular atrophy in the pathogenesis of chronic kidney disease progression.Pediatric nephrology (Berlin, Germany) · 2016Review
- VRACs and other ion channels and transporters in the regulation of cell volume and beyond.Nature reviews. Molecular cell biology · 2016Review
- Measurement of Rapid Amiloride-Dependent pH Changes at the Cell Surface Using a Proton-Sensitive Field-Effect Transistor.Biosensors · 2016Article
- Na+-H+ exchanger-1 (NHE1) regulation in kidney proximal tubule.Cellular and molecular life sciences : CMLS · 2015Review
- The biophysical and molecular basis of intracellular pH sensing by Na+/H+ exchanger-3.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Human-induced pluripotent stem cell-derived cardiomyocytes for studies of cardiac ion transporters.American journal of physiology. Cell physiology · 2013Article
- Differential effects of lipids and lyso-lipids on the mechanosensitivity of the mechanosensitive channels MscL and MscS.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- On the role of the difference in surface tensions involved in the allosteric regulation of NHE-1 induced by low to mild osmotic pressure, membrane tension and lipid asymmetry.Cell biochemistry and biophysics · 2012Article
- Phosphoinositide binding differentially regulates NHE1 Na+/H+ exchanger-dependent proximal tubule cell survival.The Journal of biological chemistry · 2011Article
- Programmed bending reveals dynamic mechanochemical coupling in supported lipid bilayers.PloS one · 2011Article
- Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function.The Journal of biological chemistry · 2010Article
- NHE3 activity is dependent on direct phosphoinositide binding at the N terminus of its intracellular cytosolic region.The Journal of biological chemistry · 2010Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Sodium/hydrogen exchangers (NHEs) are ubiquitous ion transporters that serve multiple cell functions. We have studied two mammalian isoforms, NHE1 (ubiquitous) and NHE3 (epithelial-specific), by measuring extracellular proton (H+) gradients during whole-cell patch clamp with perfusion of the cell interior. Maximal Na(+)-dependent H+ fluxes (JH+) are equivalent to currents >20 pA for NHE1 in Chinese hamster ovary fibroblasts, >200 pA for NHE1 in guinea pig ventricular myocytes, and 5-10 pA for NHE3 in opossum kidney cells. The fluxes are blocked by an NHE inhibitor, ethylisopropylamiloride, and are absent in NHE-deficient AP-1 cells. NHE1 activity is stable with perfusion of nonhydrolyzable ATP [adenosine 5'-(beta,gamma-imido)triphosphate], is abolished by ATP depletion (2 deoxy-D-glucose with oligomycin or perfusion of apyrase), can be restored with phosphatidylinositol 4,5-bisphosphate, and is unaffected by actin cytoskeleton disruption (latrunculin or pipette perfusion of gelsolin). NHE3 (but not NHE1) is reversibly activated by phosphatidylinositol 3,4,5-trisphosphate. Both NHE1 and NHE3 activities are disrupted in giant patches during gigaohm seal formation. NHE1 (but not NHE3) is reversibly activated by cell shrinkage, even at neutral cytoplasmic pH without ATP, and inhibited by cell swelling. NHE1 in Chinese hamster ovary fibroblasts (but not NHE3 in opossum kidney cells) is inhibited by agents that thin the membrane (L-alpha-lysophosphatidylcholine and octyl-beta-D-glucopyranoside) and activated by cholesterol enrichment, which thickens membranes. Expressed in AP-1 cells, however, NHE1 is insensitive to these agents but remains sensitive to volume changes. Thus, changes of hydrophobic mismatch can modulate NHE1 but do not underlie its volume sensitivity.
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