ArticleNucleic acids research2004
The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Redefining pleiotropic drug resistance in a pathogenic yeast: Pdr1 functions as a sensor of cellular stresses inmSphere · 2023Article
- Effect of the His-Tag Location on Decapping Scavenger Enzymes and Their Hydrolytic Activity toward Cap Analogs.ACS omega · 2020Article
- Targeting mRNA Decapping in AML.Cancer cell · 2018Article
- Proteomic Responses Under Cold Stress Reveal Unique Cold Tolerance Mechanisms in the Pacific White Shrimp (Frontiers in physiology · 2018Article
- Growth condition dependency is the major cause of non-responsiveness upon genetic perturbation.PloS one · 2017Article
- Cytoplasmic mRNA turnover and ageing.Mechanisms of ageing and development · 2015Review
- A model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymes.FEBS letters · 2013Article
- mRNA quality control pathways in Saccharomyces cerevisiae.Journal of biosciences · 2013Review
- Proposal for a method to estimate nutrient shock effects in bacteria.BMC research notes · 2012Article
- RNA degradation in Saccharomyces cerevisae.Genetics · 2012Review
- Low level genome mistranslations deregulate the transcriptome and translatome and generate proteotoxic stress in yeast.BMC biology · 2012Article
- Activation of 5'-3' exoribonuclease Xrn1 by cofactor Dcs1 is essential for mitochondrial function in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Structural requirements for Caenorhabditis elegans DcpS substrates based on fluorescence and HPLC enzyme kinetic studies.The FEBS journal · 2010Article
- Mechanistic and kinetic analysis of the DcpS scavenger decapping enzyme.The Journal of biological chemistry · 2008Article
- DcpS scavenger decapping enzyme can modulate pre-mRNA splicing.RNA (New York, N.Y.) · 2008Article
- Distributed control for recruitment, scanning and subunit joining steps of translation initiation.Nucleic acids research · 2007Article
- Subunit architecture of multimeric complexes isolated directly from cells.EMBO reports · 2006Article
- Caenorhabditis elegans decapping proteins: localization and functional analysis of Dcp1, Dcp2, and DcpS during embryogenesis.Molecular biology of the cell · 2005Article
- Scavenger decapping activity facilitates 5' to 3' mRNA decay.Molecular and cellular biology · 2005Article
- Current awareness on yeast.Yeast (Chichester, England) · 2005Article
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5 authors.
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Abstract
Dcs1, the m7GpppX pyrophosphatase of Saccharomyces cerevisiae, has been reported to 'scavenge' capped 5' end fragments generated by 3'-->5' mRNA degradation. We now show that the absence of Dcs1, and the closely related Dcs2 protein, compromises cellular responses to glucose-deprivation stress as well as to step changes in glucose availability. Dcs1 and Dcs2 form homo- and heterodimers, with the heterodimer appearing as cells enter diauxie. Despite the previously observed increase in abundance of the mRNA encoding the neutral trehalase (Nth1) in the stationary phase, the total enzyme activity of Nth1 decreases in this phase of growth. Changes in trehalase activity are significant because the non-reducing disaccharide trehalose is thought to stabilize cellular components under stress conditions. In the dcs1Delta and dcs1Deltadcs2Delta mutants, normal regulation of trehalase activity is lost. Nutrient stress induces DCS1 and DCS2 transcription via the cAMP-PKA signalling pathway. Dcs1 also becomes phosphorylated as the availability of glucose diminishes, and we test the role of this phosphorylation in the stress response. Further evidence indicates that Dcs1 plays a complementary role to the translation factor eIF4E in preventing capped 5' fragments of mRNA from interfering with translation initiation. We conclude that Dcs1 function influences cellular responses to changes in nutrient avialability, while Dcs2 seems to act as a modulator of Dcs1 function.
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