ArticleApplied and environmental microbiology2004
Simultaneous discrimination between 15 fish pathogens by using 16S ribosomal DNA PCR and DNA microarrays.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 101 citations in OpenAlex.
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- Application of Engineered Zinc Finger Proteins Immobilized on Paramagnetic Beads for Multiplexed Detection of Pathogenic DNA.Journal of microbiology and biotechnology · 2021Article
- Antibiotic Resistance and Virulence Genes ofInfection and drug resistance · 2021Article
- Identification and typing of fish pathogenic species of the genus Tenacibaculum.Applied microbiology and biotechnology · 2018Review
- Food Waste to Energy: An Overview of Sustainable Approaches for Food Waste Management and Nutrient Recycling.BioMed research international · 2017Review
- Monitoring and managing microbes in aquaculture - Towards a sustainable industry.Microbial biotechnology · 2016Review
- A review approaches to identify enteric bacterial pathogens.Jundishapur journal of microbiology · 2015Review
- Simultaneous detection of multiple fish pathogens using a naked-eye readable DNA microarray.Sensors (Basel, Switzerland) · 2012Article
- A zinc finger protein array for the visual detection of specific DNA sequences for diagnostic applications.Nucleic acids research · 2011Article
- Detection of representative enteropathogenic bacteria, Vibrio spp., pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, using a virulence factor gene-based oligonucleotide microarray.Journal of microbiology (Seoul, Korea) · 2010Article
- Identifying Fishes through DNA Barcodes and Microarrays.PloS one · 2010Article
- Development of a DNA microarray for detection of expressed equine classical MHC class I sequences in a defined population.Immunogenetics · 2010Article
- A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities.BMC microbiology · 2008Article
- In situ-synthesized virulence and marker gene biochip for detection of bacterial pathogens in water.Applied and environmental microbiology · 2008Article
- DNA microarray-based identification of serogroups and virulence gene patterns of Escherichia coli isolates associated with porcine postweaning diarrhea and edema disease.Applied and environmental microbiology · 2007Article
- High-density universal 16S rRNA microarray analysis reveals broader diversity than typical clone library when sampling the environment.Microbial ecology · 2007Article
- Application of a high-density oligonucleotide microarray approach to study bacterial population dynamics during uranium reduction and reoxidation.Applied and environmental microbiology · 2006Article
- Identifying influenza viruses with resequencing microarrays.Emerging infectious diseases · 2006Article
- Using DNA microarrays to identify library-independent markers for bacterial source tracking.Applied and environmental microbiology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a DNA microarray suitable for simultaneous detection and discrimination between multiple bacterial species based on 16S ribosomal DNA (rDNA) polymorphisms using glass slides. Microarray probes (22- to 31-mer oligonucleotides) were spotted onto Teflon-masked, epoxy-silane-derivatized glass slides using a robotic arrayer. PCR products (ca. 199 bp) were generated using biotinylated, universal primer sequences, and these products were hybridized overnight (55 degrees C) to the microarray. Targets that annealed to microarray probes were detected using a combination of Tyramide Signal Amplification and Alexa Fluor 546. This methodology permitted 100% specificity for detection of 18 microbes, 15 of which were fish pathogens. With universal 16S rDNA PCR (limited to 28 cycles), detection sensitivity for purified control DNA was equivalent to <150 genomes (675 fg), and this sensitivity was not adversely impacted either by the presence of competing bacterial DNA (1.1 x 10(6) genomes; 5 ng) or by the addition of up to 500 ng of fish DNA. Consequently, coupling 16S rDNA PCR with a microarray detector appears suitable for diagnostic detection and surveillance for commercially important fish pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.