ArticleApplied and environmental microbiology2004
Lipid accumulation, lipid body formation, and acyl coenzyme A oxidases of the yeast Yarrowia lipolytica.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 208 citations in OpenAlex.
- Lipid droplet proteome plasticity in plant evolution, growth and development, and response to stress.Journal of experimental botany · 2026Review
- A Copper-Inducible Regulatory System for Dynamic Enhancement of Gene Editing Efficiency in Yarrowia lipolytica.Biotechnology journal · 2026Article
- Multi-Omics Analysis Reveals 1-Propanol-Induced Pentadecanoic Acid Biosynthesis inBiomolecules · 2025Article
- Proteomes reveal metabolic capabilities ofmSystems · 2023Article
- Key enzymes involved in the utilization of fatty acids byFrontiers in microbiology · 2023Review
- A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Characterization of Acyl-CoA Oxidases from the Lipolytic YeastJournal of microbiology and biotechnology · 2022Article
- Enhancing very long chain fatty acids production in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Metabolic engineering strategies for de novo biosynthesis of sterols and steroids in yeast.Bioresources and bioprocessing · 2021Review
- Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous.Microbial cell factories · 2021Review
- Increasing lipid yield in Yarrowia lipolytica through phosphoketolase and phosphotransacetylase expression in a phosphofructokinase deletion strain.Biotechnology for biofuels · 2021Article
- Analysis ofMetabolic engineering communications · 2020Article
- Two-step optimization process for grass hydrolysate application as biodiesel feedstock with novel quality characteristics.Environmental science and pollution research international · 2020Article
- Catalytic and physical features of a naturally immobilized3 Biotech · 2020Article
- Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.MicrobiologyOpen · 2020Article
- Modular engineering for microbial production of carotenoids.Metabolic engineering communications · 2020Review
- Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides.Biotechnology and bioengineering · 2020Article
- An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries.Microorganisms · 2020Review
- Engineering the YeastFrontiers in bioengineering and biotechnology · 2020Article
- Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica.Microbial cell factories · 2019Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yarrowia lipolytica contains five acyl-coenzyme A oxidases (Aox), encoded by the POX1 to POX5 genes, that catalyze the limiting step of peroxisomal beta-oxidation. In this study, we analyzed morphological changes of Y. lipolytica growing in an oleic acid medium and the effect of POX deletions on lipid accumulation. Protrusions involved in the uptake of lipid droplets (LDs) from the medium were seen in electron micrographs of the surfaces of wild-type cells grown on oleic acid. The number of protrusions and surface-bound LDs increased during growth, but the sizes of the LDs decreased. The sizes of intracellular lipid bodies (LBs) and their composition depended on the POX genotype. Only a few, small, intracellular LBs were observed in the mutant expressing only Aox4p (Deltapox2 Deltapox3 Deltapox5), but strains expressing either Aox3p or both Aox3p and Aox4p had the same number of LBs as did the wild type. In contrast, strains expressing either Aox2p or both Aox2p and Aox4p formed fewer, but larger, LBs than did the wild type. The size of the LBs increased proportionately with the amount of triacylglycerols in the LBs of the mutants. In summary, Aox2p expression regulates the size of cellular triacylglycerol pools and the size and number of LBs in which these fatty acids accumulate.
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