ArticleApplied and environmental microbiology2004
Impact of heterologous expression of Escherichia coli UDP-glucose pyrophosphorylase on trehalose and glycogen synthesis in Corynebacterium glutamicum.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
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- Complete genome sequence of Arthrobacter sp. PAMC25564 and its comparative genome analysis for elucidating the role of CAZymes in cold adaptation.BMC genomics · 2021Article
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- Two Homologous Enzymes of the GalU Family inInternational journal of molecular sciences · 2019Article
- Identification and Microbial Production of the Raspberry Phenol Salidroside that Is Active against Huntington's Disease.Plant physiology · 2019Article
- Metabolic engineering of Corynebacterium glutamicum for anthocyanin production.Microbial cell factories · 2018Article
- Production of the compatible solute α-D-glucosylglycerol by metabolically engineered Corynebacterium glutamicum.Microbial cell factories · 2018Article
- On the Ancestral UDP-Glucose Pyrophosphorylase Activity of GalF from Escherichia coli.Frontiers in microbiology · 2015Article
- Trends in bacterial trehalose metabolism and significant nodes of metabolic pathway in the direction of trehalose accumulation.Microbial biotechnology · 2013Review
- Functional metagenomics reveals novel salt tolerance loci from the human gut microbiome.The ISME journal · 2012Article
- Engineering trehalose synthesis in Lactococcus lactis for improved stress tolerance.Applied and environmental microbiology · 2011Article
- Metabolic engineering of Corynebacterium glutamicum for trehalose overproduction: role of the TreYZ trehalose biosynthetic pathway.Applied and environmental microbiology · 2006Article
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4 authors.
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Abstract
Trehalose is a disaccharide with a wide range of applications in the food industry. We recently proposed a strategy for trehalose production based on improved strains of the gram-positive bacterium Corynebacterium glutamicum. This microorganism synthesizes trehalose through two major pathways, OtsBA and TreYZ, by using UDP-glucose and ADP-glucose, respectively, as the glucosyl donors. In this paper we describe improvement of the UDP-glucose supply through heterologous expression in C. glutamicum of the UDP-glucose pyrophosphorylase gene from Escherichia coli, either expressed alone or coexpressed with the E. coli ots genes (galU otsBA synthetic operon). The impact of such expression on trehalose accumulation and excretion, glycogen accumulation, and the growth pattern of new recombinant strains is described. Expression of the galU otsBA synthetic operon resulted in a sixfold increase in the accumulated and excreted trehalose relative to that in a wild-type strain. Surprisingly, single expression of galU also resulted in an increase in the accumulated trehalose. This increase in trehalose synthesis was abolished upon deletion of the TreYZ pathway. These results proved that UDP-glucose has an important role not only in the OtsBA pathway but also in the TreYZ pathway.
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