ArticleGenetics2004
Efficient transformation of the beetle Tribolium castaneum using the Minos transposable element: quantitative and qualitative analysis of genomic integration events.
Article in Genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed.
- Next-Generation Precision Breeding in Peanut (Insects · 2026Review
- Article
- Egg Cooling After Oviposition Extends the Permissive Period for Microinjection-Mediated Genome Modification inInternational journal of molecular sciences · 2024Article
- The white gene as a transgenesis marker for the cricket Gryllus bimaculatus.G3 (Bethesda, Md.) · 2024Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms.Biology open · 2023Article
- Generating and testing the efficacy of transgenic Cas9 in Tribolium castaneum.Insect molecular biology · 2022Article
- Review
- Experimental duplication of bilaterian body axes in spider embryos: Holm's organizer and self-regulation of embryonic fields.Development genes and evolution · 2020Review
- Article
- Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos.Journal of visualized experiments : JoVE · 2017Article
- Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods.Frontiers in physiology · 2017Review
- Non-lethal genotyping of Tribolium castaneum adults using genomic DNA extracted from wing tissue.PloS one · 2017Article
- Article
- Biochemical characterization and comparison of two closely related active mariner transposases.Biochemistry · 2014Article
- Cell and tissue dynamics during Tribolium embryogenesis revealed by versatile fluorescence labeling approaches.Development (Cambridge, England) · 2013Article
- Heat shock-mediated misexpression of genes in the beetle Tribolium castaneum.Development genes and evolution · 2012Article
- Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes.Genetics · 2012Article
- MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes.Nature methods · 2011Article
- The evolution of dorsal-ventral patterning mechanisms in insects.Genes & development · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic transformation in insects holds great promise as a tool for genetic manipulation in species of particular scientific, economic, or medical interest. A number of transposable elements have been tested recently as potential vectors for transformation in a range of insects. Minos is one of the most promising elements because it appears to be active in diverse species and has the capacity to carry large inserts. We report here the use of the Minos element as a transformation vector in the red flour beetle Tribolium castaneum (Coleoptera), an important species for comparative developmental and pest management studies. Transgenic G(1) beetles were recovered from 32.4% of fertile G(0)'s injected with a plasmid carrying a 3xP3-EGFP-marked transposon and in vitro synthesized mRNA encoding the Minos transposase. This transformation efficiency is 2.8-fold higher than that observed when using a plasmid helper. Molecular and genetic analyses show that several independent insertions can be recovered from a single injected parent, but that the majority of transformed individuals carry single Minos insertions. These results establish Minos as one of the most efficient vectors for genetic transformation in insects. In combination with piggyBac-based transgenesis, our work allows the introduction of sophisticated multicomponent genetic tools in Tribolium.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.